Lyo-Ready LAMP/RT-LAMP Mixes
Meridian’s Lyo-Ready LAMP/RT-LAMP Mixes are ready-to-use, 4x concentrated, fully optimized mixes for isothermal amplification methods such as Loop mediated isothermal amplification (LAMP), designed to dramatically speed up assay development time and reduce time to results.
Loop mediated isothermal amplification is a highly sensitive nucleic acid amplification method that has gained popularity in recent years due to its advantages over qPCR, such as quicker time to results, simple assay workflow, and point-of-care usability. However, the initial assay design and development can be more complex with 4 to 6 primers which are used in combination to generate a robust, fast, and sensitive result. Through extensive R&D research, Meridian has discovered a blend of bst DNA polymerase, reverse transcriptase, dNTPs, Mg2+, excipients, and stabilizers that are used with customer-selected primers and probes for creating any custom DNA or RNA LAMP test. Experimental data on both RNA and DNA templates demonstrate that the mixes are up to 2x faster than other suppliers, generating results in half the time. In addition, the mixes incorporate Meridian’s Lyo-Ready technology and can be used in a liquid format or lyophilized to create an ambient-temperature stable assay (please see the product guide and FAQs for recommendations on lyophilization parameters).
By using a ready-to-use master mix such as Lyo-Ready LAMP Mix or Lyo-Ready RT-LAMP Mix, LAMP test assay development is faster, simpler, and more efficient, speeding up your product development time and allowing you to commercialize your new products faster.
Can also be used wet
Lyo-Ready RT-LAMP 1-Step Mix, 4x, MDX108
不含甘油的混合液，适用于 RT-LAMP 等等等温应用，含有 Bst DNA 聚合酶、逆转录酶、反应缓冲液、dNTPs 和 lyo-兼容辅料，是开发常温 LAMP 检测的理想之选。
文档与资源
The Lyo-Ready LAMP Mixes are 4x master mix formulated with excipients optimized for freeze-drying to create ambient-temperature stable assays. Given cold chain management is a major limiting factor in resource-limited settings, ambient-temperature stable point-of-care tests (POCT) offer several advantages, including no refrigeration, reduced shipping, and handling costs, and extended shelf-life. By using Meridian’s Lyo-Ready LAMP Mixes, the process of developing a sensitive and high-performing assay is made significantly easier, with the added flexibility of using the mix in a wet or dried-down format.
产品资料
开发常温稳定检测的分子试剂可冻干LAMP试剂
开发常温稳定检测的分子试剂可冻干RT-LAMP试剂
FAQs: Lyo-Ready LAMP Mixes
环介导等温扩增与qPCR的主要区别在于，在环介导等温扩增中，扩增是在恒温下完成的，而在qPCR中，扩增需要在一个热循环器上在多个温度之间循环。 环介导等温扩增使用具有高链置换活性的聚合酶，如Bst DNA聚合酶，由于LAMP反应不需要通过多个温度的变化，因此可以在短时间内完成快速的扩增。
Lyo-Ready LAMP and Lyo-Ready RT-LAMP 1-Step Mix can both be used directly as a liquid mix, or lyophilized, without changing the sensitivity of the assay. The master mixes are 4x concentration, this means that in a 20 L reaction, 5 L the master mix and 1 L of primers and probes are used allowing for up to 14 L of sample to be added, however if the master mix, primers, and probes are lyophilized, then up to 20 L of sample can be added.
Yes, it is possible to designed a multiplex LAMP test using different primers and probes, however they can be difficult to design and optimize as each sequence requires three sets of primers.
Yes, the Lyo-Ready LAMP and Lyo-Ready RT-LAMP 1-Step Mix can be used as a liquid mix or lyophilized and stored for up to 24 months without needing to change the reaction conditions and this drying/ambient temperature storage will not affect the sensitivity of the POCT.
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