Glycerol-Free T4 Polynucleotide Kinase (HC)
T4 Polynucleotide Kinase is widely used to phosphorylate the 5โ end of both DNA and RNA during labeling and ligation by catalyzing the transfer of the ฮณ-phosphate from ATP to the 5ยด hydroxyl of single stranded and double stranded DNA, RNA, and nucleoside 3ยด monophosphates. T4 Polynucleotide Kinase also catalyzes the hydrolytic removal of the 3ยด phosphate from 3ยด-phosphoryl polynucleotides, deoxyribonucleoside 3ยด monophosphates, and deoxyribonucleoside-3ยด,5ยด-diphosphates to form a 3ยด-hydroxyl group.
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Meridian's Glycerol-Free T4 Polynucleotide Kinase (HC)
- 5' phosphorylation for subsequent ligation for NGS
- End-labeling for probes and oligodeoxynucleotide primers
- Removes 3โฒ phosphoryl groups from DNA and RNA
- Glycerol-free, for lyophilization and ambient temperature storage
Glycerol-Free T4 Polynucleotide Kinase (HC), MDX206
Glycerol-free enzyme used to phosphorylate the ends of both DNA and RNA during labeling and ligation, mainly used in next-generation sequencing (NGS) library preparation. Ideal for lyophilization.
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Description
Glycerol-Free T4 Polynucleotide Kinase (HC) is supplied at 20 U/ยตL and is mainly used in next-generation sequencing (NGS) library preparation, where it can be used separately or as part of an End-Repair Mix, where phosphates are added to the 5โฒ-hydroxyl terminus of double stranded DNA for subsequent ligation of adaptors by T4 DNA Ligase.ย Glycerol-Free T4 Polynucleotide Kinase (HC) is in a glycerol-free storage buffer and is supplied with a Lyo-Ready reaction buffer and has been optimized to deliver excellent, stable performance without glycerol, allowing you to deliver reliable results with the added capability for lyophilization. This gives you the flexibility to confidently store mixtures at ambient temperature or produce diagnostic assays that rely on miniaturized reaction components, such as point-of-care NGS.
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FAQs: Glycerol-Free T4 Polynucleotide Kinase
Can I use T4 Polynucleotide Kinase and T4 DNA Ligase in the same reaction?
Yes, but not for radioactive labelling reactions as the non-radioactive ATP from the T4 Ligase buffer will interfere.
Can I inactivate T4 Polynucleotide Kinase?
Yes, incubation for 20 minutes at 65ยฐC completely inactivates T4 Polynucleotide Kinase.
What are the advantages of a high-concentration enzyme?
High-concentration enzymes help reduce volumes when drying down, making the process faster, they also reduce volumes in microfluidic, cartridges and point of care (POC) devices, allowing for more sample to be added. They facilitate optimization and can speed up the reaction (as long as there is substrate available to bind to), increasing sensitivity and yield, as a result, they can be employed in a wide range of assay formats, allowing more flexibility for development and cost saving during commercialization.
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