Lyo-Ready AOF dUTP DNA and RNA/DNA qPCR Urine
Urine Mixes are glycerol-free, inhibitor-tolerant formulations optimized for high-throughput qPCR applications. Made from animal-origin-free (AOF) ingredients, these mixes are specifically designed to tolerate/withstand carryover contamination, ensuring reproducible results. They are highly effective with both crude and purified urine samples, making them versatile and reliable for a variety of diagnostic applications.
Key Product Features
- Highly sensitive detection of RNA/DNA, even in crude whole blood, serum and plasma samples
- Compatible with lyophilization for developing ambient-temperature stable assays
- Incorporates dUTP, making it ideal for high-throughput and multiplex assays
- Animal origin-free and sustainable, designed to reduce environmental impact while aligning with future eco-regulations
Description
Lyo-Ready AOF dUTP DNA qPCR Urine (MDX352) uses an advanced one-tube formulation, incorporating the latest innovations in buffer chemistry and PCR enhancers, along with an optimized concentration of recombinant antibody-mediated hot-start polymerase, dNTPs (including dUTP), and MgCl2. Lyo-Ready AOF dUTP RNA/DNA qPCR Urine, (MDX353) also includes reverse transcriptase and RNase inhibitor for comprehensive nucleic acid detection.
Lyo-Ready AOF dUTP DNA/RNA qPCR mixes utilize the dUTP/UDGase system to prevent carryover contamination, minimizing false positives and ensuring accurate results, especially in high-throughput workflows. BSA-free and sustainable, these mixes also align with evolving regulatory trends toward in vitro systems, reducing reliance on animal-sourced reagents while offering cost-effective, room-temperature stability through their lyophilization-ready formulation.
Urine, a versatile and non-invasive clinical specimen, is increasingly used for diagnosing infectious diseases, metabolic disorders, cancer, and drug monitoring. However, inhibitors like urea and nucleases can impact accuracy. Our inhibitor-tolerant Lyo-Ready mixes ensure sensitive RNA/DNA detection, even in crude urine samples, providing reliable results for diverse diagnostic applications.
Key points:
- Supports the development of urine-based screening assays, such as assays detecting sexually transmitted diseases (STDs) or urinary tract infections (UTIs)
- Enables highly sensitive detection of low-copy number bacteria, viruses, and cell-free nucleic acids directly from crude urine samples.
- Facilitates quick and accurate identification of slow-growing and hard-to-culture microorganisms, reducing ambiguous results.
- Offers the flexibility to remain as a liquid mix or be lyophilized, creating ambient-temperature stable assays with extended shelf life.
Example Protocol
| Reagent Name | Volume |
|---|---|
| Lyo-Ready AOF Direct DNA qPCR Urine, 4x | 5 µL |
| Primer-Probe Mix, 20x | 1 µL |
| Water | up to 10 µL |
Figure 1. Lyo-Ready AOF Direct DNA qPCR Urine. Combine reagents as shown for a total reaction volume of 20 μL. Briefly vortex and spin down briefly in a microcentrifuge. Include the reaction for 2 minutes at 95°C and then cycle for 5 seconds at 95°C and 230 seconds at 60°C for 45 cycles.
Lyo-Ready AOF dUTP DNA qPCR Urine, MDX352
An optimized qPCR master mix tolerant to inhibitors in urine. Perfect for multiplexing, high throughput assays where there is a greater risk of false positives from cross-over contamination.
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FAQs: qPCR Urine Test
What does Animal origin–free (AOF) mean?
Does not contain materials derived from eukaryotic animals.
Is UDGase supplied in the mixes?
No, UDGase (Uracil DNA Glycosylase) needs to be added separately where there is a greater risk of false positives from cross-over contamination.
What are the advantages of using urine over other sample types?
The advantages of a quantitative PCR urine test are that it is completely non-invasive, and it can be self-sampled in large quantities. Urine testing is used in the diagnosis and management of infectious diseases (including STDs), hormone and metabolic disorders, renal diseases, bladder cancer, urinary tract infections (UTIs) and for monitoring recreational drug use.
What types of infectious diseases can urine detect?
The most common infections are urinary tract infections (UTIs), most commonly Escherichia coli, Protus mirabilis, Enterococcus faecalis, Staphylococcus saprophyticus and Klebsiella pneumoniae. Several other infections can be diagnosed by urine DNA and urine RNA tests including community-acquired pneumonia (CAP), legionellosis, tuberculosis, congenital cytomegalovirus (CMV) infection, and arboviruses, such as Chikungunya virus, dengue virus and Zika virus. Parasites can also be diagnosed from urine testing for the detection of eggs from Schistosoma haematobium. Furthermore, urine has been used for screening of different sexually transmitted diseases (STD) such as Neisseria gonorrhoeae and Chlamydia trachomatis.
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