qPCR offers speed, sensitivity, and a strong regulatory track record—ideal for targeted applications like companion diagnostics, liquid biopsy, MRD testing, and treatment selection.

It enables high-sensitivity detection even in low-input samples like plasma-derived ctDNA, integrates easily into automated, high-throughput workflows, and delivers results within hours. qPCR offers speed, sensitivity, and a strong regulatory track record—ideal for targeted applications like companion diagnostics, liquid biopsy, MRD testing, and treatment selection.

Used in FDA-approved diagnostics for:

• EGFR – NSCLC
• BRAF – melanoma
• KRAS – colorectal cancer

Targeted next-generation sequencing (tNGS) enables simultaneous analysis of dozens to hundreds of genes—offering broader coverage than single-target assays. Commonly used in tumor profiling, MRD monitoring, and therapy selection, tNGS strikes a balance between depth and practicality in liquid biopsy and panel-based testing.

Two main approaches:

• Amplicon-based – fast, focused, and cost-effective
• Hybrid-capture – greater sensitivity and detection of complex variants

Meridian supports amplicon-based workflows with modular, glycerol-free reagents for sensitive amplification, efficient clean-up, and precise quantification—reducing hands-on time and improving assay consistency.
From amplification through to library prep, our solutions integrate seamlessly into your pipeline:

Whole genome sequencing (WGS) provides a comprehensive, base-by-base view of the entire genome – capturing all variant types, including SNVs, indels, CNVs, structural variants, and non-coding mutations.

WGS requires no prior mutation knowledge, making it valuable for rare, treatment-resistant, or complex cancers. It’s used in translational research, clinical trials, and national precision medicine programs, with strong relevance in pediatric and hematologic oncology.

While powerful, WGS adoption is limited by cost, turnaround time, and the need for advanced bioinformatics.
NGS library preparation is a key step, directly impacting data quality, yield, and consistency. With limited or low-quality samples, efficient library prep is essential to maximize usable data and ensure reliable variant detection—especially in challenging applications like FFPE or liquid biopsy.