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Liquid Stable Flex RNA/DNA qPCR Mix

A high-concentration liquid one-step RT-qPCR master mix with long-term ambient stability – no cold chain, drying or lyophilization required. Supplied without primers and probes, MDX988 enables assay developers to configure assays at the point of use while benefiting from up to 10 months’ stability at 20 °C, 1 month at 37 °C or 12 months at 4 °C.
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Key Product Features

  • 10-Month Ambient Stability - Full performance at 20 °C; no refrigeration or cold shipping.

  • Flexible Assay Design - Add primers and probes at the point of use.

  • Bench-Stable After Assembly - Stable for 4 hours at ambient temperature once oligos are added.

  • Automation-Compatible - Works with manual and automated liquid handling platforms.

  • Consistent Performance - High sensitivity and specificity for singleplex, multiplex and fast-cycling RT-qPCR.

  • Built-In Contamination Control - Includes dUTP for UDG/UNG-compatible workflows.

Description

Meridian’s Liquid Stable Flex RNA/DNA qPCR Mix (MDX988) is a high-concentration (5x), liquid one-step RT-qPCR master mix that delivers consistent, reliable results without the need for cold storage. Designed for flexibility, it can be stored and transported at ambient temperature for up to 10 months, helping assay developers reduce costs, simplify logistics and support more sustainable molecular testing workflows.

Supplied without primers and probes, MDX988 gives end users the freedom to add their own oligonucleotides at the point of use. This makes one master mix suitable for multiple targets, panels and development paths while avoiding the cold-chain burden of conventional frozen RT-qPCR reagents.

Its liquid, ready-to-use format is fully compatible with manual pipetting and automated liquid handling, from small-scale assay development to high-throughput molecular diagnostic workflows.

Key Product Benefits:

  • Cold Chain Not Required
  • Flexible Assay Design
  • Simplified Inventory Management
  • No Drying, Lyophilization or Rehydration Required
  • Rapid Setup at Point-of-Use
  • Supports Automation and High-Throughput Processing
  • Reduced Storage and Shipping Costs
  • Lower Environmental Impact
  • Supports Multiplex and Fast-Cycling RT-qPCR
  • Rapid Assay Setup

Liquid Stable Flex RNA/DNA qPCR Mix, MDX988

Application: qPCRSpecimen Type: DNAConcentration: 5xHigh Concentration: YesWet: Yes
Glycerol-Free: YesLyo-Ready: YesAir-Dryable: NoDryable: Lyo-ReadySustainability: Yes

One-step RT-qPCR master mix supplied without primers and probes, providing up to 10 months of ambient-temperature stability for the master mix without refrigeration or lyophilization.

Available in 0.8 mL (200 reactions), 4 mL (1,000 reactions) and 40 mL (10,000 reactions) aliquots
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Product Data

Ambient Temperature Stability for up to 10 Months

PCR amplification curves showing ΔRn increasing with Cycle for blue and red lines; curves rise steeply after cycle ~22 and plateau near 100k–175k.
Line graph of qPCR amplification curves plotting ΔRn (vertical) against Cycle (horizontal); multiple blue and red curves rise sharply around cycles 28–32, then plateau near 125,000–150,000.
Line chart of ΔRn vs Cycle for multiple samples (blue and red curves) showing amplification increasing after cycle ~25.
Liquid Stable Flex RNA/DNA qPCR Mix (MDX988) was stored at –20°C (control) or ambient temperature at 20°C for 10 months and then tested in triplex RT-qPCR assay with two template dilutions. These results indicate that compared to a frozen master mix, the Liquid Stable Flex RNA/DNA qPCR Mix stored at ambient temperature showed no significant loss in performance, as measured by fluorescence intensity and Ct values, demonstrating that Liquid Flex Ready RNA/DNA qPCR Mix is stable at ambient temperature for up to 10 months.

High performance and reproducibility

Bar chart of mean Ct values for three targets, comparing two reagents (MDX988 and Vazyme) with error bars; y-axis Mean Ct 20–40.
Bar chart titled '15 pg total RNA' comparing mean Ct values for three targets; for each target, two bars (MDX988 and Vazyme) with error bars indicating variability, showing a side-by-side comparison across targets.
Performance of MDX988 was evaluated against Vazyme’s AccurSTART II U+ One Step RT-qPCR Super Premix in a triplex RT-qPCR assay using two input concentrations of mammalian total RNA (1.5 ng and 15 pg). MDX998 demonstrated earlier Ct values across the three targets, indicating greater sensitivity. In addition, MDX988 showed lower standard deviation between replicates, highlighting superior reproducibility and overall assay performance compared to the competitor formulation. 

Catalogs & Brochures

Reagent Solutions for Molecular Diagnostics

Reagent Solutions for Molecular Diagnostics Catalog

Air-Dryable qPCR/RT-qPCR Catalog

Air-Dryable qPCR/RT-qPCRCatalog

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Liquid Stable Ready RNA/DNA qPCR Mix

FAQs: Liquid Stable Flex RNA/DNA qPCR Mix

How should assays be prepared?
MDX988 is supplied without primers and probes. Add the appropriate primers, probes and template at the point of use according to the Product Handling Guide. Use sterile plasticware and nuclease-free reagents. If oligonucleotides need to be rehydrated, use nuclease-free sterile water and handle assembled reactions under sterile conditions, for example, using a laminar flow hood.
Yes. Once primers and probes are added, assembled reactions are bench-stable for up to 4 hours at ambient temperature. For long-term ambient storage of assays preassembled with primers and probes, use a ready-format product such as Liquid Stable Ready RNA/DNA qPCR Mix (MDX998).
Yes. Shipping and temporary storage for up to 10 months at 20 °C has no detrimental effect on the quality of Liquid Stable Flex RNA/DNA qPCR Mix when handled according to recommended guidelines. Transit and storage time should be considered when determining remaining self-life.
qPCR can amplify tiny amounts of DNA, so even small amounts of contamination can produce false positives. Contamination can arise from other samples, the laboratory environment or carryover amplification products from previous reactions. MDX988 includes dUTP for workflows designed to incorporate uracil into amplification products and can support UDG/UNG-compatible carryover-control strategies. 
Shipping and temporary storage for up to 10 months at ambient temperature, 20 °C, has no detrimental effect on product quality. For long-term storage, store at -20 °C and avoid repeated freeze/thaw cycles. 
The master mix without primers and probes maintains performance for up to 10 months at 20 °C, 1 month at 37 °C or 12 months at 4 °C. When stored and handled under recommended conditions, full activity of the mix without primers and probes is retained until the expiry date printed on the label.
Yes. MDX988 is supplied in a liquid format and is suitable for manual pipetting, automated dispensing and low- to high-throughput liquid handling workflows.
Choose MDX988 Flex when the customer needs one ambient-stable RT-qPCR backbone that can support multiple assays, targets or panels by adding primers and probes at the point of use. Choose a Ready-format product when primers and probes need to be preassembled into the assay for long-term ambient storage and deployment.
Ambient-stable liquid formulations can help simplify storage, reduce cold-chain shipping requirements, avoid drying and rehydration steps, support high-throughput workflows and improve deployment flexibility while maintaining RT-qPCR performance. 

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Have questions about a product? Want to learn more about Meridian’s molecular or immunoassay reagent portfolio? We want to hear from you!

Meridian’s Liquid Ambient Stable Master Mixes & qPCR Reagents offer 12-month ambient stability. Save on costs, reduce waste and ship easily—no cold chain or special storage needed. Learn how our new liquid qPCR master mixes can help you!

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