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Revolutionize your assay development with the only liquid DNA qPCR master mix offering true long-term ambient stability – even when pre-assembled.
While other liquid mixes require cold-chain logistics or offer only limited shelf life, Meridian’s Liquid Stable Ready DNA qPCR Mix (5x) is engineered to maintain performance for at least 12 months at 15 – 25 °C, with or without primers and probes added. This eliminates the need for cold storage, lyophilization or air-drying – without compromising sensitivity, speed or reliability.
Designed for modern, high-throughput workflows, it gives assay developers the freedom to simplify logistics, scale up confidently and develop assays that are ready to deploy – anywhere.
Meridian’s Liquid Stable Ready DNA qPCR Mix (5x) (MDX364) is a high-concentration, liquid ambient-stable master mix that delivers consistent amplification performance throughout its entire shelf life – even under fast cycling and multiplex conditions. While other mixes may degrade over time, especially when stored at ambient temperatures, this formulation maintains performance from day one.
With no refrigeration, lyophilization, or rehydration required, complete reactions can be stored and shipped at ambient temperatures, minimizing cold chain costs, workflow complexity, and environmental impact.
Key Product Benefits:
qPCR master mix ready to receive the primers and probes needed to form a test with 12-month ambient temperature stability, no refrigeration or lyophilization is required.
Liquid Stable Flex DNA qPCR Mix (MDX364) was stored at –20°C (blue) or ambient temperature (22°C) (red) for 12 months and then tested in triplex assays with two template dilutions. These results indicate that compared to a frozen master mix stored at –20°C (control), the Liquid Stable Ready DNA qPCR Mix stored at ambient temperature for 12 months showed no significant loss in performance, as measured by fluorescence intensity and Ct values, demonstrating that Liquid Stable Ready DNA qPCR Mix is stable at ambient temperature for at least 12 months.
Liquid Stable Flex DNA qPCR Mix (MDX364) was stored at –20°C (blue) or ambient temperature (22°C) (red) for 12 months with primers and probe and then tested in a triplex assay. Results show no significant difference in fluorescence intensity or Ct values compared to the frozen control, confirming that MDX364 pre-assembled with oligonucleotides remains stable at ambient temperature for at least 12 months.
Meridian Liquid Stable Ready DNA qPCR Mix (MDX364)
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Since the master mix and the premixed assays can be stored at ambient temperature for at least one year, it is critical to use sterile plasticware and reagents (i.e., tubes, oligonucleotides). If needed, we recommend rehydrating the required oligonucleotides in nuclease-free, sterile water.
The assembled assays (Assay Ready DNA qPCR Mix, primers and probes) should be handled under sterile conditions, for example, using a laminar flow hood.
The ready-to-use assays dispensed in tubes should be sealed with air-tight caps to prevent evaporation. We recommend storing them away from light to avoid bleaching the fluorophores that may be used.
As qPCR can amplify tiny amounts of DNA, preventing contamination is essential, as even small amounts of qPCR contamination can produce false positives. Cross-over qPCR contamination can include cross-contamination from other samples, contamination from elsewhere in the laboratory, and carryover qPCR contamination from amplification products and primers used in prior qPCR experiments. The latter causes most of the false positive results seen in qPCR. Preventing contamination can be difficult and so laboratory procedures must be in place to ensure DNA from one assay is not re-amplified in subsequent assay.
Shipping and temporary storage for up to 12 months at ambient temperatures (15−25°C) has no detrimental effects on the quality of Liquid Stable Ready DNA qPCR Mix. However, for long-term storage we do recommend storing at -20°C. Repeated freeze/thaw cycles should be avoided.
When stored and handled under the recommended conditions, full activity of the reagents is retained until the Expiry Date printed on the label.
Have questions about a product? Want to learn more about Meridian’s molecular or immunoassay reagent portfolio? We want to hear from you!
Meridian’s Liquid Ambient Stable Master Mixes & qPCR Reagents offer 12-month ambient stability. Save on costs, reduce waste and ship easily—no cold chain or special storage needed. Learn how our new liquid qPCR master mixes can help you!
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| Specimen Type |
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| Application |
| Catalog |
| Sizes |
| Wet(Liquid) |
| Air-Dryable |
| Glycerol-Free |
| Lyo-Ready |
| Concentration |
| High Concentration |
| Description |
| Request Sample |
