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Liquid Stable Ready RNA/DNA qPCR Mix

Meridian’s Liquid Stable Ready RNA/DNA qPCR Mix is a one-step RT-qPCR master mix formulated for long-term ambient storage.

Designed for modern molecular workflows, MDX998 enables assays to be pre-assembled with primers and probes and stored at ambient temperature for up to 10 months without lyophilization, drying or rehydration. The mix maintains performance for up to 10 months at 20 °C, 1 month at 37 °C or 12 months at 4 °C, with or without primers and probes added.

By reducing cold-chain requirements and simplifying assay preparation, MDX998 helps assay developers streamline manufacturing, simplify logistics and accelerate deployment of ready-to-use molecular diagnostic assays.

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Key Product Features

  • 10-Month Ambient Stability - Store fully assembled assays at 20°C for up to 10 months.

  • No Lyophilization Required - Eliminate drying, lyophilization, and rehydration steps.

  • High Sensitivity & Specificity - Reliable RT-qPCR performance across a broad dynamic range.

  • Contamination Control - Includes dUTP for UDG/UNG-compatible workflows.

Description

Meridian’s Liquid Stable Ready RNA/DNA qPCR Mix is a high-concentration, ambient-stable liquid one-step RT-qPCR master mix designed for molecular assay development and manufacturing.

The formulation delivers consistent amplification performance throughout its shelf life and supports standard, fast cycling and multiplex RT-qPCR workflows. Assays assembled with primers and probes can be stored and shipped at ambient temperature without freezing, drying, lyophilization or rehydration.

By reducing cold-chain requirements and simplifying handling, MDX998 helps lower workflow complexity, improve manufacturing flexibility, support automation and reduce the environmental impact associated with temperature-controlled shipping and storage.

Key Product Features:

  • Cold Chain Not Required
  • Supports Assay-Ready Workflows
  • Simplified Manufacturing and Deployment
  • Supports Automation and High-Throughput Processing
  • Lower Environmental Impact
  • Reduced Storage and Shipping Costs
  • Simplified Inventory Management
  • Faster Time to Market

Liquid Stable Ready RNA/DNA qPCR Mix, 5x, MDX998

Application: qPCRSpecimen Type: DNAConcentration: 4xHigh Concentration: YesWet: Yes
Glycerol-Free: YesLyo-Ready: YesAir-Dryable: NoDryable: Lyo-ReadySustainability: Yes

RT-qPCR master mix designed to receive the primers and probes, providing up to10 months of ambient stability, without refrigeration or lyophilization.

Available in 0.8 mL (200 reactions), 4 mL (1,000 reactions) and 40 mL (10,000 reactions) aliquots
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Product Data

Ambient Temperature Stability with Oligos for up to 10 Months

Amplification plot with ΔRn on the y-axis and cycle on the x-axis; blue and red curves rise as amplification occurs in multiple samples.
Amplification plot from qPCR showing blue and red amplification curves rising after ~28 cycles, indicating target detection; axes labeled Cycle and ΔRn.
Amplification plot showing ΔRn vs cycle with blue and red curves rising after cycle ~20.

Liquid Stable Ready RNA/DNA qPCR Mix (MDX998) was stored at –20°C (control) or ambient temperature at 20°C for 10 months and then tested in triplex RT-qPCR assay with two template dilutions. These results indicate that compared to a frozen master mix, the Liquid Stable Ready RNA/DNA qPCR Mix stored at ambient temperature showed no significant loss in performance, as measured by fluorescence intensity and Ct values, demonstrating that Liquid Stable Ready RNA/DNA qPCR Mix is stable at ambient temperature for up to 10 months.

Sensitivity

qPCR amplification curves: multiple blue sigmoidal lines of ΔRn rising with cycle (2–50), plateauing around 80,000–85,000.
Liquid Stable Ready RNA/DNA qPCR Mix, 5x (MDX998) was used in a 10-fold serial dilution of viral RNA target (10,000, 1,000, 100, 10 and 1 copy, respectively). The results illustrate that MDX998 offers exceptional sensitivity (down to 1 copy per reaction), reproducibility and qPCR performance.

Catalogs & Brochures

Meridian Liquid Stable Ready DNA qPCR Mix

Meridian Liquid Stable Ready DNA qPCR Mix

Reagent Solutions for Molecular Diagnostics

Reagent Solutions for Molecular DiagnosticsCatalog

Lyo-Ready & Air-Dryable LAMP & RT-LAMP Master Mixes

Lyo-Ready & Air-Dryable LAMP & RT-LAMP Master Mixes

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FAQs: Liquid Stable Ready RNA/DNA qPCR Mix

How should the premixed assay be prepared?
Since the master mix and the premixed assays can be stored at ambient temperature for at least 10 months, it is critical to use sterile plasticware and reagents (i.e. tubes, oligonucleotides). If needed, we recommend rehydrating the required oligonucleotides in nuclease-free, sterile water. The recommended volume of primers and probes to be added to MDX998 is described in the Product Handling Guide. The assembled assays (Assay Ready RNA/DNA qPCR Mix, primers and probes) should be handled under sterile conditions, for example, using a laminar flow hood. The ready-to-use assays dispensed in tubes should be sealed with air-tight caps to prevent evaporation. We recommend storing them away from light to avoid bleaching the fluorophores that may be used. 
Yes. Assays assembled with MDX998, primers, and probes can be stored and shipped at ambient temperature when handled according to recommended guidelines. Because shipping time contributes to the overall validated shelf life, customers should consider transit and storage conditions when determining the remaining shelf life available to end users. For applications requiring the maximum available shelf life upon receipt, refrigerated or frozen shipping may be preferred.
As qPCR can amplify tiny amounts of DNA, preventing contamination is essential, as even small amounts of qPCR contamination can produce false positives. Cross-over qPCR contamination can include cross-contamination from other samples, contamination from elsewhere in the laboratory, and carryover qPCR contamination from amplification products. The latter causes most of the false positive results seen in qPCR. Preventing contamination can be difficult and so laboratory procedures must be in place to ensure DNA from one assay is not re-amplified in subsequent assays.
Shipping and temporary storage for up to 10 months at ambient temperatures (20°C) has no detrimental effects on the quality of Liquid Stable Ready RNA/DNA qPCR Mix. However, for long-term storage we do recommend storing at -20°C. Repeated freeze/thaw cycles should be avoided.
When stored and handled under the recommended conditions, full activity of the mix (without the primers and probes) is retained until the Expiry Date printed on the label. 
Assays prepared with MDX998, primers, and probes can be stored at 20°C for up to 10 months while maintaining RT-qPCR performance. This enables the development of ready-to-use assay formats without requiring lyophilization, drying, or rehydration.
Yes. MDX998 is supplied in a liquid format and is suitable for automated dispensing, assay assembly, and high-throughput manufacturing workflows.
Ambient-stable formulations can help simplify storage, reduce cold-chain shipping requirements, support ready-to-use assay formats, and improve manufacturing flexibility while maintaining RT-qPCR performance. This can help reduce operational complexity and support broader assay deployment.

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