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qPCR Master Mixes

The ready-to-use qPCR and RT-qPCR master mixes have been developed for fast cycling and are designed for superior sensitivity and specificity with probe-detection technology. A combination of the latest advances in buffer chemistry and PCR enhancers, in conjunction with an antibody or chemical-mediated DNA polymerase promote rapid, consistently accurate detection of the intended targets, even with multiplex qPCR assays.
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Name Specimen Type Application Catalog Order a Sample Sizes Description Wet(Liquid) Air-Dryable Dryable Glycerol-Free Lyo-Ready Concentration High Concentration Detail Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready dUTP 1-Step RT-qPCR Mix Lyo-Ready Mix, Lyo-Ready Mix RT-qPCR MDX113 Order a Sample Available in 10 mL (1,000 Rxns) or 100 mL (10,000 Rxns) aliquots

Glycerol-free mix containing Taq polymerase, RNase Inhibitor, reaction buffer, dNTP/dUTP mix, MgCl2 and lyo-excipients and separate reverse transcriptase , developed to remove background PCR product contamination.

Yes No Lyo-Ready YesYes Yes 2x No
Inhibitor-Tolerant RT-qPCR Lo-ROX Mix, 4x Blood, saliva, urine, stool, tissue RT-qPCR MDX105 Order a Sample Available in 5 mL (500 Rxns) or 50 mL (10,000 Rxns) aliquots

Combination of the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with high quality enzymes, dNTPs and MgCl2, for use in the presence of inhibitors.

Yes No No NoNo No 4x Yes
Air-Dryable 1-Step RT-qPCR Mix, 4x Air-Dryable Mix RT-qPCR MDX095 Order a Sample Available in 5 mL (500 Rxns) or 50 mL (10,000 Rxns) aliquots

Glycerol-free RT-qPCR mix containing Taq polymerase, reverse transcriptase, reaction buffer, dNTPs, MgCl2 and air-dry compatible excipients, ideal for developing ambient temperature, multiplex molecular assays.

Yes Yes Air-Dryable YesYes No 4x Yes
Air-Dryable qPCR Mix, 4x PCR, qPCR, Air-Dryable Mix qPCR MDX082 Order a Sample Available in 5 mL (500 Rxns) or 50 mL (10,000 Rxns) aliquots

Glycerol-free qPCR mix containing Taq polymerase, reaction buffer, dNTPs, MgCl2 and air-dry compatible excipients, ideal for developing ambient temperature, multiplex molecular assays.

Yes Yes Air-Dryable YesYes No 4x Yes
Inhibitor-Tolerant qPCR Mix, 5x PCR, qPCR qPCR MDX073 Order a Sample Available in 2 mL (500 Rxns) or 40 mL (10,000 Rxns) aliquots

Combination of the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with high quality enzymes, dNTPs and MgCl2, for use in the presence of inhibitors.

Yes No No NoNo No 5x Yes
Fast qPCR Mix, 5x PCR, qPCR qPCR MDX072 Order a Sample Available in 2 mL (500 Rxns) or 40 mL (10,000 Rxns) aliquots

The latest advances in buffer chemistry and PCR enhancers and stabilizers, together with a hot-start polymerase, dNTPs and MgCl2, for highly reproducible, accurate assay results under fast thermal cycling conditions.

Yes No No NoNo No 5x Yes
Lyo-Ready 1-Step RT-qPCR Virus Mix Lyo-Ready Mix, Lyo-Ready Mix RT-qPCR MDX062 Order a Sample Available in 10 mL (1,000 Rxns) or 100 mL (10,000 Rxns) aliquots

Taq polymerase, RNase inhibitor, reaction buffer, dNTP, MgCl2 and lyo-excipients and separate reverse transcriptase, optimized for amplification of RNA with high secondary structure.

Yes No Lyo-Ready YesYes Yes 2x No
Hi-throughput dUTP qPCR Lo-ROX PCR, qPCR, dU Containing qPCR MDX060 Order a Sample Available in 10 mL (1,000 Rxns) or 100 mL (10,000 Rxns) aliquots

Contains Taq polymerase, reaction buffer, dNTP, MgCl2, stabilizers, PCR enhancers, and ROX dye. The dTTP has been substituted for dUTP, so that UDGase can be added to remove background PCR carryover product contamination.

Yes No No NoNo No 2x No
Fast 1-Step RT-qPCR Mix RT-PCR, RT-qPCR RT-qPCR MDX032 Order a Sample Available in 5 mL (500 Rxns) or 100 mL (10,000 Rxns) aliquots

A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start polymerase, dNTPs and MgCl2 and separate reverse transcriptas, ideal for fast, accurate, highly reproducible RT-qPCR.

Yes No No NoNo No 2x No
NGS Library Quantification PCR, qPCR NGS MDX039 Order a Sample Available in 500 Rxns

Fast qPCR Mix, pre-diluted DNA standards, primer mix and dilution buffer, designed to accurately measure the number of amplifiable DNA fragments in Illumina-based NGS libraries before loading into a flow cell.

Yes No No NoNo No 2x No
Hi-throughput dUTP qPCR Mix PCR, qPCR, dU Containing qPCR MDX031 Order a Sample Available in 5 mL (500 Rxns) or 100 mL (10,000 Rxns) aliquots

qPCR mix containing Taq polymerase, reaction buffer, dNTP/dUTP mix and MgCl2, developed to remove background PCR product contamination.

Yes No No NoNo No 2x No
Low DNA qPCR Mix PCR, qPCR qPCR MDX030 Order a Sample Available in 5 mL (500 Rxns) or 100 mL (10,000 Rxns) aliquots

Highly purified qPCR master mix with stringent chemical hot-start properties and low bioburden, ideal for PCR of low-copy bacterial targets to avoid false-positive amplification.

Yes No No NoNo No 2x No
Low LOD 1-Step RT-qPCR Mix RT-PCR, RT-qPCR RT-qPCR MDX025 Order a Sample Available in 10 mL (1,000 Rxns) or 100 mL (10,000 Rxns) aliquots.

RT-qPCR mix a dNTP/dUTP mix, designed for stringent amplification of highly structured and very low copy-number RNA and DNA targets.

Yes No No NoNo No 2x No
Lyo-Ready 1-Step RT-qPCR Mix Lyo-Ready Mix RT-qPCR MDX024 Order a Sample Available in 10 mL (1,000 Rxns) or 100 mL (10,000 Rxns) aliquots

Glycerol-free Taq polymerase, reaction buffer, dNTP, MgCl2, and lyo-excipients, and separate thermostable reverse transcriptase, ideal for developing room temperature, lyophilized, multiplex qPCR assays.

Yes No Lyo-Ready YesYes Yes 2x No
High-Specificity Pfu HS Mix PCR, qPCR NGS MDX006 Order a Sample Available in 0.025 mL (500 Units) or 1 mL (20,000 Units) aliquots

High concentration, high-fidelity Taq DNA polymerase used for the amplification of a next-generation sequencing (NGS) library.

Yes No No NoNo No 2x No
Lyo-Ready qPCR Mix 2.6x PCR, qPCR, Lyo-Ready Mix qPCR MDX023 Order a Sample Available in 8 mL (500 Rxns) or 100 mL (12,500 Rxns) aliquots

Glycerol-free Taq polymerase, reaction buffer, dNTP, MgCl2, and lyo-excipients, ideal for developing room temperature, lyophilized, multiplex qPCR assays.

Yes No Lyo-Ready YesYes Yes 2.6x No
Lyo-Ready qPCR Mix PCR, qPCR, Lyo-Ready Mix qPCR MDX021 Order a Sample Available in 5 mL (500 Rxns) or 100 mL (10,000 Rxns) aliquots

Glycerol-free Taq polymerase, reaction buffer, dNTP, MgCl2, and lyo-excipients, ideal for developing room temperature, lyophilized, multiplex qPCR assays.

Yes No Lyo-Ready YesYes Yes 2x No
Fast qPCR Mix PCR, qPCR qPCR MDX020 Order a Sample Available in 5 mL (500 Rxns) or 100 mL (10,000 Rxns) aliquots

The latest advances in buffer chemistry and PCR enhancers and stabilizers, together with a hot-start polymerase, dNTPs and MgCl2, for highly reproducible, accurate assay results under fast thermal cycling conditions.

Yes No No NoNo No 2x No
Inhibitor-Tolerant RT-qPCR Mix, 4x Blood, saliva, urine, stool, tissue, RT-PCR, RT-qPCR RT-qPCR MDX016 Order a Sample Available in 5 mL (500 Rxns) or 50 mL (10,000 Rxns) aliquots

Universal, high concentration, inhibitor tolerant RT-qPCR master mix, for direct amplification from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood, sputum, saliva and stool.

Yes No No NoNo No 4x No
Inhibitor-Tolerant qPCR Mix Blood, saliva, urine, stool, tissue qPCR MDX013 Order a Sample Available in 5 mL (500 Rxns) or 100 mL (10,000 Rxns) aliquots

Universal inhibitor tolerant qPCR master mix, for direct amplification from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood, sputum, saliva and stool.

Yes No No NoNo No 2x No

Not seeing what you’re looking for? Inquire about a new product

Lyophilization and Air-Dryable Ready Master Mixes

Lyophilization and air-dryable ready options allow room temperature shipping and storage, extended shelf-life, and increased flexibility in sample volume and so sensitivity. For reduction in the background and even greater sensitivity we also have dU options that are ideal for multiplex assays on open, automated, high-throughput instruments. These products are also available as components in order to fit the development of your molecular diagnostic tests.

Why We Are Second To None

Meridian offers diagnostic manufacturers the complete solution with over 3,000 antigens and antibodies covering more than 500 diseases and an extensive range of specialty qPCR/RT-qPCR master mixes and molecular enzymes all manufactured under ISO13485 regulations.

  • Path Copy

    Large Sample Size

  • Lot Reservation

    Lot Reservation

  • ISO13485

    ISO13485

  • World-Class Customer Service

    World-Class Customer Service

Get In Touch With A Specialist

Have questions about a product? Want to learn more about Meridian’s molecular or immunoassay reagent portfolio? We want to hear from you!


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