Glycerol-Free Taq HS High Conc. DNA Polymerase
Glycerol-Free Taq HS High Conc. (50 U/µL) has been optimized to deliver excellent, stable performance without glycerol, meaning it is also compatible with lyophilization, as a stand-alone enzyme, or when combined with additional assay components in a reaction mix.
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Meridian's Glycerol-Free Taq HS High Conc. DNA Polymerase
- High concentration of Taq DNA Polymerase allowing for concentrated bulk production of PCR master mixes.
- Highly concentrated Taq DNA Polymerase allows for concentrated bulk production of PCR master mixes
- Retains the same reproducible performance as with conventional formats.
- Glycerol-free solution, ideal for preparation of dried-down PCR tests.
Efficient and sensitive amplification with both wet (black) and lyophilized (blue) PCR tests
A 10-fold serial dilution of template DNA was used to set up two sets of qPCR assays. One set was lyophilized (blue) and the other left as a wet mix (black). Results illustrate that lyophilization of Glycerol-Free Taq HS High Conc. does not have an effect on the quality of the qPCR, as both the assays demonstrate identical efficiency and sensitivity.
Glycerol-Free Taq HS High Conc. DNA Polymerase, MDX170
Glycerol-free, high concentration, high fidelity DNA polymerase with separate dilution and reaction buffer, ideal for NGS library amplification and gene expression. Ideal for lyophilization.
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Description
Glycerol-Free Taq HS High Conc. is supplied as a blend of high‑performance glycerol‑free Taq DNA Polymerase with a Taq hot-start antibody and is suitable for difficult, multiplex PCR that require fast amplification, whilst maintaining both high specificity and sensitivity.
Glycerol-Free Taq HS High Conc. is ideal for PCR kit manufactures and PCR molecular test facilities, requiring large amounts of DNA polymerases. The highly concentrated Glycerol-Free Taq HS High Conc. allows for concentrated bulk production of PCR master mixes and the glycerol free format is useful when using automated pipetting robots and other applications where accurate pipetting of small volumes is crucial.
Specifications
| Description | A lyophilization-compatible Taq DNA polymerase with Taq antibody, suitable for difficult, multiplex molecular tests that require fast amplification, whilst maintaining both high specificity and sensitivity. |
| Concentration | Between 50 and 60 U/µL (see CoA) |
| Purity | >50 % (as a percentage of total protein) |
| Glycerol content | <0.02 % |
| Appearance | Clear, colorless solution |
| Hot Start | Antibody mediated |
| Application | PCR, real-time PCR, two-step RT-qPCR |
| Sample type | cDNA, DNA |
| Presentation | 3 vials |
| Storage | -20 °C |
| Mix stability | See outer label |
| DNA Contamination | None detected in PCR amplification with traces overlay with the negative control on E. coli and mouse genomic DNA specific targets. |
| DNase Contamination | No detectable degradation |
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FAQs: Glycerol-Free Taq HS High Conc.
How is Glycerol-Free Taq HS High Conc. supplied?
Glycerol-Free Taq HS High Conc. is supplied as a glycerol-free blend of Taq DNA polymerase with Taq hot-start antibody at 50 U/μL. We also have MDX011 Glycerol-Free Taq HS 50 U/µL that has the glycerol‑free Taq DNA polymerase and a separate glycerol‑free Taq antibody and enzyme dilution buffer, to allow greater flexibility in developing PCR tests.
What are the advantages of a 10x DNA polymerase?
Glycerol-Free Taq HS High Conc. (50 U/µL) is ideal for PCR kit manufactures and DNA testing facilities, requiring large amounts of DNA polymerases. The highly concentrated enzyme allows for concentrated bulk production of PCR master mixes.
Concentrated bulks in glycerol-free formats are also attractive when using automated pipetting robots and other applications where accurate pipetting of small volumes is crucial, as glycerol makes the solution more viscous.
Concentrated PCR master mixes are easier to lyophilize due to the reduced volume of the concentrated mix.
What are the storage conditions and stabilities of Glycerol-Free Taq HS High Conc.?
Glycerol is a cryoprotectant and is normally part of the storage buffer, where it serves to protect Taq DNA polymerase during freezing conditions. The high concentration of Glycerol-Free Taq HS 50 U/µL means that you get the same protection to freeze/thawing as a DNA polymerase that contains glycerol.
After drying for how long will the lyophilized PCR tests stay stable to use?
A lyophilized PCR molecular test will remain stable for up to 2 years at ambient temperature if correctly stored in sealed pouches.
Can Glycerol-Free Taq HS High Conc. be used to create quantitative PCR tests?
Yes, Glycerol-Free Taq HS High Conc. is ideal for any multiplex PCR assays, particularly quantitative PCR molecular tests that require fast amplification, whilst maintaining both high specificity and sensitivity.
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