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Join our in-house experts William Ferreira, Ph.D. and Steve Hawkins, Ph.D., for their latest webinar where they share their insights on the newest range of Lyo-Ready Genotyping Direct qPCR Master mixes that support commercial assays designed to detect mutations.
In this presentation, you will learn how to detect single nucleotide polymorphisms (SNPs) directly from blood, plasma, urine, stool or FFPE tissue (down to 1 copy/reaction) using a simplified workflow that eliminates the need for nucleic acid extraction.
Speakers:
William Ferreira, Ph.D., R&D Team Leader
Steve Hawkins, Ph.D., Product Marketing Manager
DNA genotyping is a powerful technology that allows the identification of genetic variations that can lead to an increased risk of developing certain diseases. Additionally, genotyping has a fundamental role in companion diagnostics, as it enables the characterization of patients’ genetic profiles that can be used to guide treatment decisions.
Here, we will introduce two novel master mixes developed to provide PCR-based identification of mutations directly from the clinical samples, such as blood, plasma or FFPE tissue, bypassing DNA extraction and streamlining the analysis of both liquid and tissue biopsies.
Speakers:
Mihai-Nicolae Podaru, PhD (R&D Senior Scientist, Meridian Bioscience)
Moderator:
Steve Hawkins, PhD (Product Marketing Manager, Meridian Bioscience)
As the IVD industry continues to evolve, new regulations frequently come into place to better control an innovative and growing industry. Molecular IVDs in particular are experiencing tighter regulations regarding the presence of appropriate controls within the assay. All tests must include both positive and negative controls which are subjected to the whole testing process, including the extraction step. Novel co-extracted exogenous controls are providing cutting edge solutions for monitoring RNA or DNA analysis including the detection of potential inhibition. These controls can be used across a variety of sample types and assays, representing a significant advantage over traditional spike-in controls.
Speakers:
Michele Amasio, Ph.D. (Research and Development Senior Manager, Meridian Bioscience)
Moderator:
Steve Hawkins, PhD (Product Marketing Manager, Meridian Bioscience)
qPCR and RT-qPCR are widely used in the development of molecular diagnostic assays with applications in human diagnostics, animal health, environmental, plant and food testing.
Lyophilization is a common technique used for creating ambient temperature stable PCR assays. However, the process is time consuming, complex to optimize and requires expensive equipment. Meridian’s new air-dryable technology replaces lyophilization with a fast, simple, and inexpensive oven-drying process, making it ideal for manufacturing room-temperature stable, highly sensitive and cost-effective molecular diagnostic assays.
This webinar will cover:
Presenters:
Dr Steve Hawkins – Product Marketing Manager
Dr Michele Amasio – Research and Development Manager
Molecular point of care (POC) tests are rapidly gaining traction in the diagnostic market, especially for infectious diseases. While LAMP and qPCR testing share common advantages such as quick turnaround time and high sensitivity and specificity, LAMP has additional benefits, making it possible for testing to be carried out essentially anywhere – from an airport to school, to a remote medical clinic.
In this webinar you will learn about:
Speakers:
Elda Iljazi, Ph.D. – R&D Senior Scientist, Meridian Bioscience
Dr. Michele Amasio – Research and Development Manager
Traditionally, qPCR and RT-qPCR diagnostic assays include DNA/RNA extraction and purification steps to remove inhibitors from the PCR reactions, to meet the assay specificity and sensitivity. However, these steps are laborious, expensive, and time-consuming. To overcome this, Meridian has developed inhibitor tolerant mixes specifically for blood, saliva, urine, and stool. Meridian’s Direct Sample-Specific qPCR Mixes are the first mixes on the market overcoming the limitations of inhibitors inherent in the various clinical specimens, for ultimate assay sensitivity and specificity.
In this webinar our speakers will discuss:
The optimization strategy and methodology that have led to the development of these specimen-specific mixes, which have superior performance compared to other products on the market and enable faster, more sensitive, and cost-effective diagnostic assays.
Speakers:
Dr. Rachel Currier- Acting Team Leader, Research & Development
Dr. Thomas Fouqueau- Acting Team Leader, Research & Development
Moderator
Dr. Steve Hawkins, Product Marketing Manager
Direct qPCR has many advantages over traditional qPCR workflows that involve DNA/RNA extraction. However, achieving high sensitivity with direct qPCR using whole blood samples has been challenging due to the presence of qPCR inhibitors within the sample (such as heme, salts, and proteins), and preservatives or anti-coagulants that are added during the sample collection process. The inhibitors can negatively impact the performance of the enzymes used in qPCR and RT-qPCR and they limit the amount of sample that can be added to the reaction, overall reducing the assay sensitivity and accuracy. In this talk, we will be discussing Meridian’s novel solution to overcome PCR inhibitors in direct qPCR and RT-qPCR, enabling greater sensitivity for direct detection from whole blood, improving assay reproducibility, and reducing assay development time.
Presenters
Thomas Fouqueau, PhD
Acting Team Leader, R&D, Meridian Life Science
Rachel Currier, PhD
Acting Team Leader, R&D, Meridian Life Science
Liquid biopsy is a non-invasive alternative to tissue biopsy based on ultra-sensitive detection of tumor-derived material such as ctRNA and ctDNA from biofluids, including blood, urine, stool, saliva, and sputum. qPCR based liquid biopsy possesses several advantages in cost and throughput, when compared to other technologies like NGS and ddPCR. In this talk, we will discuss Meridian’s new advances in inhibitor tolerant qPCR based liquid biopsy, that reduces the cost and complexity of these assays, while enabling ultra-sensitive detection from very low concentration cancer markers.
Presenters
Steve Hawkins
Ph.D. – Product Marketing Manager
Michele Amasio, Ph.D.
Research and Development Senior Manager
The COVID-19 pandemic accelerated the adoption of point-of-care molecular technologies that offer quick sample to results, high sensitivity, lower cost and widespread accessibility.
This webinar will cover new market-leading reagent solutions for loop-mediated isothermal amplification (LAMP) assays, which simplify assay development, speed up assay turnaround time, and deliver highly sensitive and specific amplification without DNA or RNA extraction.
Presenters
Elda Iljazi Ph.D.
Senior Scientist Meridian Bioscience
The molecular diagnostic approach for infectious disease such as STDs, tropical, and respiratory disease has seen significant development in the past twelve months, driven by a demand for better, faster, higher sensitivity assays, that can detect asymptomatic carriers. Traditionally, lab-based tests using blood samples have been the mainstay for molecular testing, however home-based, extraction-free tests, using alternative sample types (saliva, urine, stool) are now highly sought after, but pose significant challenges in assay development for both lab-based and point of care (POC) applications.
In this webinar you will learn about:
Presenters
Michele Amasio, Ph.D.
Research and Development Senior Manager
Steve Hawkins
Ph.D. – Product Marketing Manager
Meridian offers diagnostic manufacturers the complete solution with over 3,000 antigens and antibodies covering more than 500 diseases and an extensive range of specialty qPCR/RT-qPCR master mixes and molecular enzymes all manufactured under ISO13485 regulations.
Have questions about a product? Want to learn more about Meridian’s molecular or immunoassay reagent portfolio? We want to hear from you!
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