Glycerol-Free HS Tth DNA Polymerase (HC)
Thermus thermophilus (Tth) DNA Polymerase is a thermostable DNA polymerase with intrinsic reverse transcriptase (RT) activity. The enzyme is a highly processive 5′-3′ DNA polymerase and lacks 3′-5′ exonuclease activity (proof reading). It possesses a very efficient intrinsic reverse transcriptase (RT) activity in the presence of manganese ions and unlike other RT enzymes, it is not associated with RNase H activity.
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Maintains Thermostability at 95°C for 10 Minutes
Performance of Glycerol-Free HS Tth DNA Polymerase (MDX205, red), an MMLV-RT (blue) and an AMV-RT (black) after pre-incubation from 40°C to 95°C for 10 mins, in a multiplex one-step RT-qPCR assay on mouse RNA. ΔCt values were calculated against the Ct value produced by the same enzyme stored at -20 °C. The data illustrates that MDX205 maintains its full performance even after 10 min at 95°C, in contrast to MMLV-RT and AMV-RT which are inactivated above 55°C and 60°C, respectively.
Glycerol-Free Tth DNA Polymerase (HC), MDX205
Available in 4,000 Unit or 100,000 Unit aliquots
Glycerol-Free Tth DNA Polymerase (HC) is a high concentration (200 U/µL) thermostable DNA polymerase with intrinsic reverse transcriptase (RT) activity. The elevated temperatures of Tth DNA Polymerase activity overcomes the problems posed by RNA secondary structure. Resulting cDNA can be amplified by PCR using the same enzyme in the presence of Mg2+ ions. The ability of Tth DNA Polymerase to perform both reverse transcription and DNA amplification at elevated temperatures allows this enzyme to be used for quantitative RT-PCR, cloning, and gene expression analysis of cellular and viral RNA.
Glycerol-Free HS Tth DNA Polymerase uses antibody-mediated hot start technology that enhances the specificity and sensitivity of PCR. It is supplied with a 5x Reaction Buffer that contains dNTPs and excipients required for lyophilization. Glycerol-Free HS Tth DNA Polymerase enables flexible and scalable reaction volumes and is suitable for applications such as fast RT-qPCR amplification of difficult templates from crude samples.
Catalogs & Brochures
Glycerol-Free Tth DNA Polymerase (HC)Glycerol-Free Tth DNA Polymerase (HC)Catalog
Glycerol‑Free Taq HSGlycerol-Free Taq HSCatalog
Thermostable MMLV-RT55C MMLV-RTCatalog
A Comprehensive Guide to Essential Reagents for High-Throughput Molecular Diagnostic Assays (MDx)Accelerating Efficiency: A Comprehensive Guide to Essential Reagents for High-Throughput Molecular Diagnostic Assays (MDx)
FAQs: Glycerol-Free Tth DNA Polymerase (HC)
Tth DNA Polymerase is a thermostable DNA Polymerase with intrinsic reverse transcriptase activity for RT-PCR amplification. Thia allows one-step PCR or RT-PCR at much higher temperatures, overcoming problems typically associated with the high degree of secondary structure present in RNA.
Tth Polymerase error rate is 3.0 x 10-5 so it is very similar to Taq DNA polymerase.
Temperature optimum for elongation with Mg2+ is approximately +72°C and for reverse transcription with Mn2+.is approximately +60 to +70°C
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