RNase Inhibitor

Ribonucleases (RNases) are ubiquitous and can be introduced into experiments in many ways: for example co-purification during RNA isolation, carryover from bare hands and pipette tips. This RNase contamination can often go unnoticed. RNase Inhibitor is ideal for RNA-sensitive applications such as RT-qPCR as even a small amount of RNase can be detrimental to the final experimental outcome.

RNase Inhibitor is a highly efficient inhibitor of a broad spectrum of eukaryotic RNases and shows no inhibition of polymerase or reverse transcriptase activity, so can be used in cDNA synthesis or one-step PCR or quantitative PCR reactions.

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RNase Inhibitor, MDX056

Available in 250 µL (10,000 Units) or 2.5 mL (100,000 Units) aliquots

RNase Inhibitor is a recombinant protein that inhibits different RNases (A, B, C) by binding non-covalently in a 1:1 ratio. With an association constant of 1014 M, RNase Inhibitor is useful in any applications where the presence of RNases is a potential problem. RNase Inhibitor is tested for activity, SDS-PAGE purity, and the absence of endonucleases, nickases, and exonuclease.

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