Glycerol-Free Non-Bac Taq HS Polymerase (HC)

Meridian Bioscience’s high-concentration Glycerol-Free Non-Bac Taq HS Polymerase is designed for sensitive PCR and multiplex applications, ensuring accurate detection even at ultra-low target copy numbers. Produced using a recombinant eukaryotic expression system, it is completely free of E. coli DNA, eliminating false positives from bacterial contamination.

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Key Product Features

Sensitive detection of Bacterial DNA

The activity of the Glycerol-Free Non-Bac HS Taq Polymerase (HC) was tested in a triplex qPCR assay targeting marker genes from three bacteria (16s rRNA gene from Enterococcus, Mip gene from Legionella pneumophila and ToxA gene from Pseudomonas aeruginosa), using 10-fold serial dilution of the bacterial genomic DNAs. The result shows that Glycerol-Free Non-Bac HS Taq Polymerase (HC) can successfully detect multiple targets down to 1 copy of DNA in multiplex assays.

Glycerol-Free Non-Bac Taq HS Polymerase (HC), MDX252

Application: PCRSpecimen Type: DNAConcentration: 50 µg/mLHigh Concentration: YesWet: Yes

Glycerol-Free: YesLyo-Ready: Requires ExcipientsAir-Dryable: Requires ExcipientsDryable: Requires excipientsSustainability: Yes

Lyophilization-compatible, high concentration, polymerase, designed to ensuring accurate detection of low-abundance bacterial targets.

Available in 0.02 mL (1,000 Units), 0.5 mL (25,000 Units) or 5 mL (250,000 Units) aliquots

Documents & Resources

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Description

Glycerol-Free Non-Bac Taq HS Polymerase (HC) is a high concentration (50 U/µL) thermostable DNA polymerase and is supplied as a high-performance glycerol-free Taq DNA Polymerase premixed with a Taq hot start antibody. Ideal for sensitive or multiplex PCR, delivering high specificity and zero bacterial DNA.

The glycerol-free formulation can be used to produce lyophilized or air-dried, ambient-temperature stable PCR or qPCR reagents and exhibits the same high performance before and after drying, offering greater flexibility for assay design and format.

Catalogs & Brochures

Glycerol-Free Taq HS Glycerol-Free Taq HS

A Comprehensive Guide to Essential Reagents for High-Throughput Molecular Diagnostic Assays (MDx)Accelerating Efficiency: A Comprehensive Guide to Essential Reagents for High-Throughput Molecular Diagnostic Assays (MDx)

Thermostable MMLV-RT55C MMLV-RT
Catalog

FAQs: Glycerol-Free Non-Bac Taq HS Polymerase (HC)

What does “Non-Bac” mean?

“Non-Bac” refers to the enzyme’s non-bacterial expression system. Unlike traditional Taq polymerase produced in E. coli, this polymerase is expressed in a recombinant eukaryotic system, ensuring it is free from bacterial (E. coli) DNA contamination.

Why is bacterial DNA contamination a problem in PCR?

Trace bacterial DNA can act as an unintended template, leading to false positives, particularly in assays targeting bacterial or microbial DNA. This is especially critical for low-copy detection, multiplex PCR and environmental or diagnostic testing.

Is Glycerol-Free Non-Bac Taq HS Polymerase (HC) suitable for multiplex PCR?

Yes. Glycerol-Free Non-Bac Taq HS Polymerase (HC) is specifically designed for difficult and multiplex PCR applications, delivering high specificity and sensitivity even when amplifying multiple low-abundance targets in the same reaction.

Why is glycerol-free formulation important?

Glycerol-free enzymes are better suited for lyophilization and ambient storage, enabling cold-chain-free shipping and supporting point-of-care and decentralized testing applications.

Is there a difference between the polymerase with glycerol and glycerol-free formulation?

No, the Glycerol-Free Non-Bac Taq HS Polymerase (HC) can be used in exactly the same way as a glycerol-containing enzyme, however it allows the assay to be future-proofed, should you consider lyophilization and ambient storage, enabling cold-chain-free shipping and supporting point-of-care and decentralized testing applications.

What types of applications is Glycerol-Free Non-Bac Taq HS Polymerase (HC) best suited for?

It is ideal for applications where contamination control and sensitivity are critical, including:

Pathogen and infectious disease diagnostics

Environmental and water testing

Pharmaceutical and food safety screening

Point-of-care and near-patient testing

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