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A 10-fold serial dilution of template DNA was used to set up two sets of qPCR assays. One set was lyophilized (blue) and the other left as a wet mix (black). Results illustrate that lyophilization of Glycerol-Free Taq HS does not have an effect on the quality of the qPCR, as both the assays demonstrate identical efficiency and sensitivity.
Lyophilization-compatible high-performance Taq DNA polymerase, separate Taq antibody and enzyme dilution buffer, suitable for difficult, multiplex tests that require fast amplification, whilst maintaining high specificity and sensitivity.
Glycerol-Free Taq HS 50U/µL is supplied as a separate 10x, high‑performance glycerol‑free Taq DNA Polymerase, glycerol‑free Taq antibody, and glycerol‑free enzyme dilution buffer. Separating these components allows greater flexibility in assay development, making Glycerol-Free Taq HS 50 U/µL suitable for difficult, multiplex PCR that require fast amplification, whilst maintaining both high specificity and sensitivity.
Glycerol-Free Taq HS 50U/µL is ideal for PCR kit manufactures and PCR molecular test facilities, requiring large amounts of DNA polymerases. The highly concentrated Glycerol-Free Taq HS 50U/µL allows for concentrated bulk production of PCR master mixes and the glycerol free format is useful when using automated pipetting robots and other applications where accurate pipetting of small volumes is crucial.
| Description | A lyophilization-compatible Taq DNA polymerase, separate Taq antibody and enzyme dilution buffer, suitable for difficult, multiplex molecular tests that require fast amplification, whilst maintaining both high specificity and sensitivity. |
| Concentration | Between 50 and 60 U/µL (see CoA) |
| Purity | >50 % (as a percentage of total protein) |
| Glycerol content | <0.02 % |
| Appearance | Clear, colorless solution |
| Hot Start | Antibody mediated |
| Application | PCR, real-time PCR, two-step RT-qPCR |
| Sample type | cDNA, DNA |
| Presentation | 3 vials |
| Storage | -20 °C |
| Mix stability | See outer label |
| Consistency | ±0.5 Ct variance between test and reference sample |
| DNA Contamination | None detected in PCR amplification with traces overlay with the negative control on E. coli and mouse genomic DNA specific targets. |
| DNase Contamination | No detectable degradation |
Glycerol-Free Taq HS 50U/µL is supplied as a separate 50 U/μL, high‑performance glycerol‑free Taq DNA polymerase, glycerol‑free Taq antibody and glycerol‑free enzyme dilution buffer. Separating these components allows greater flexibility in developing PCR tests.
Glycerol-Free Taq HS 50 U/µL is ideal for PCR kit manufactures and DNA testing facilities, requiring large amounts of DNA polymerases. The highly concentrated Glycerol-Free Taq HS 50 U/µL allows for concentrated bulk production of PCR master mixes.
Concentrated bulks in glycerol-free formats are also attractive when using automated pipetting robots and other applications where accurate pipetting of small volumes is crucial, as glycerol makes the solution more viscous.
Concentrated PCR master mixes are easier to lyophilize due to the reduced volume of the concentrated mix.
Glycerol is a cryoprotectant and is normally part of the storage buffer, where it serves to protect Taq DNA polymerase during freezing conditions. The high concentration of Glycerol-Free Taq HS 50 U/µL means that you get the same protection to freeze/thawing as a DNA polymerase that contains glycerol.
A lyophilized PCR molecular test will remain stable for up to 2 years at ambient temperature if correctly stored in sealed pouches.
Yes, Glycerol-Free Taq HS 50 U/µL is ideal for any multiplex PCR assays, particularly quantitative PCR molecular tests that require fast amplification, whilst maintaining both high specificity and sensitivity.
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