High Conc. Glycerol-Free Bst
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Meridian's High Conc. Glycerol-Free Bst
- Optimized for isothermal amplification such as LAMP test.
- Designed for fast polymerization and so faster time to results.
- Supplied with a reaction buffer and enzyme dilution buffer.
- Ideal for creating lyophilized POCT assays.
High Conc. Glycerol-Free Bst, MDX018
High concentration glycerol-free DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification).
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Description
Specifications
| Description | High concentration glycerol-free DNA polymerase (exonuclease minus), with strand-displacement properties. Bst DNA Polymerase is used for Isothermal DNA amplification and LAMP (Loop-mediated Isothermal Amplification). |
| Concentration | >100 units/µL measured against a standard |
| Appearance | Clear, colorless solution |
| Application | Loop-mediated isothermal amplification |
| Sample type | cDNA, DNA |
| Presentation | 3 vials |
| Storage | -20 °C |
| Enzyme Stability | See outer label |
| Assay Stability | Up to 24 months at ambient temperature following air-drying or lyophilization |
| DNase/RNase Contamination | No detectable degradation |
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FAQs: High Conc. Glycerol-Free Bst
How is isothermal amplification different from qPCR?
The main difference between loop mediated isothermal amplification (LAMP) and qPCR is that in a LAMP test, the amplification is achieved using a single constant temperature, while in the qPCR, amplification requires cycling between multiple temperatures on a thermocycler. Instead of melting DNA strands apart at high temperatures, loop mediated isothermal amplification uses polymerases with high strand displacement activity, like Bst DNA polymerases. As these polymerases do not need multiple temperatures, they can amplify a target in less than an hour, and in some cases in as little time as 10 minutes.
Is Meridian’s Bst DNA Polymerase warm/hot start?
No Meridian’s Bst DNA Polymerase does not come with warm or hot start; instead, the reaction buffer does not contain magnesium and so the enzyme is completely inactive until magnesium is added. If you are concerned about setting your reaction up at room temperature, we recommend adding the magnesium last, just before running the experiment and it will prevent non-specific amplification.
Why is Meridian’s Glycerol-Free Bst DNA Polymerase only sold at high-concentration?
Repeated freeze-thawing of Bst DNA polymerase will damage the enzyme due to crystal formation and so glycerol is normally added to prevent this crystal formation. Making the Bst high concentration also stops crystal formation, so that the glycerol can be removed, and this allows the Bst to be lyophilized.
Can LAMP be used for a multiplex assay?
Yes, it is possible to design a multiplex LAMP test using different primers and probes, however they can be difficult to design and optimize as each sequence requires three sets of primers.
Can Glycerol-Free Bst DNA Polymerase be used in place of other DNA polymerases for thermal cycling?
No, the reaction temperatures are too high for enzyme stability.
Does Glycerol-Free Bst DNA Polymerase have reverse transcriptase activity?
Yes, Glycerol-Free Bst DNA Polymerase does have some reverse transcriptase activity, however for robust and consistent reactions we recommend the addition of a reverse transcriptase.
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