Lyo-Ready AOF dUTP DNA LAMP
Lyo-Ready AOF dUTP DNA LAMP is a glycerol-free, ready-to-use, 4x concentrated, fully optimized mix for isothermal amplification methods such as loop-mediated isothermal amplification (LAMP), designed to speed up assay development time and reduce time to results. The glycerol-free mix contains optimized excipients compatible with freezing and lyophilization, making it ideal for point-of-care testing (POCT). Made from animal-origin-free (AOF) ingredients, this mix is specifically designed to prevent carryover contamination, ensuring reproducible results.
Key Product Features
Description
Lyo-Ready AOF dUTP DNA LAMP combines the latest advances in buffer chemistry together with an optimized concentration of a strand-displacing DNA polymerase, and proprietary lyo-excipients allowing ambient temperature stabilization through lyophilization. Lyo-Ready AOF dUTP DNA LAMP contains dUTP and has been designed for fast, sensitive and highly reproducible DNA target amplification. In order to produce lyophilized ambient-temperature stable LAMP assays, specific primers need to be added to Lyo-Ready AOF dUTP DNA LAMP prior to lyophilization. Lyo-Ready AOF dUTP DNA LAMP does not contain animal-derived components, aligning with evolving regulatory trends toward more sustainable in vitro systems, reducing reliance on animals while offering cost-effective, room-temperature stability through a lyophilization-ready formulation.Key points:
- Ideal for point of care diagnostic platforms.
- Reduce risk of carryover contamination.
- Concentrated 4x master mix.
- Contains all the required excipients for subsequent lyophilization.
Example Protocol
| Reagent Name | Volume | Final Concentration (1x) |
|---|---|---|
| Lyo-Ready AOF dUTP DNA LAMP, 4x | 6.25 µL | 1x |
| MgSO4 (100 mM) (to be added to the mix) | 2 μL | 8 mM |
| FIP/BIP Primers (25x) | 1 μL | 1.6 µM* |
| F3/B3 Primers (25x) | 1 μL | 0.2 µM* |
| Loop F/B Primers (25x) | 1 μL | 0.8 µM* |
| Sample DNA | Variable | > 10 copies |
| Water (ddH2O) | to 25 µL |
Lyo-Ready AOF dUTP DNA LAMP, MDX301
Glycerol-free mix for isothermal applications such as LAMP, containing Bst DNA polymerase, reaction buffer, dNTPs and lyo excipients. Perfect for LAMP assays where there is a greater risk of false positives from cross-over contamination.
Documents & Resources
Catalogs & Brochures
Related Products
FAQs: Lyo-Ready AOF dUTP DNA LAMP
How is isothermal amplification different from qPCR?
The key difference between loop-mediated isothermal amplification (LAMP) and quantitative PCR (qPCR) lies in how DNA amplification is achieved. LAMP operates at a constant temperature, eliminating the need for thermal cycling. In contrast, qPCR requires precise cycling through multiple temperature stages using a thermocycler.
Instead of using high temperatures to denature DNA, LAMP relies on specialized polymerases, such as Bst DNA polymerase, that have strong strand displacement activity. This allows DNA amplification to occur rapidly and efficiently at a single temperature. As a result, LAMP can produce detectable amounts of DNA in under an hour, and sometimes in as little as 10 minutes.
Can LAMP be used for a multiplex assay?
Yes, it is possible to design a multiplex LAMP test using different primers and probes.
Are reaction conditions the same for lyophilized and liquid mixes?
Yes, the Lyo-Ready LAMP and Lyo-Ready RT-LAMP 1-Step Mixes (including Lyo-Ready AOF dUTP DNA LAMP) can be used as a liquid mix or lyophilized and stored for up to 24 months without affecting the sensitivity of the tests.
Get In Touch With A Specialist
Have questions about a product? Want to learn more about Meridian’s molecular or immunoassay reagent portfolio? We want to hear from you!
By submitting your information in this form, you agree that your personal information may be stored and processed in any country where we have facilities or service providers, and by using our “Contact Us” page you agree to the transfer of information to countries outside of your country of residence, including to the United States, which may provide for different data protection rules than in your country. The information you submit will be governed by our Privacy Statement.