Lyophilized NGS Library Preparation Kit
Our Lyophilized NGS Library Preparation Kit is a ready-to-use complete solution for sequencing-by-synthesis (SBS) protocols. It removes the need for cold chain shipping and storage and increases the accessibility of NGS reagents and applications.

Lyophilized NGS Library Preparation Kit
Lyophilized reactions in tubes, packed in sealed pouches for ambient temperature stability

Three tubes consisting of the lyophilized components for end-repair and A-tailing, ligation, and library amplification. These can be stored at ambient temperature and are ready to use, just add DNA solution.
Workflow

The straightforward design ensures that the NGS library preparation process is fast and convenient, contributing to cost-effective, sustainable, and accessible NGS technology.
Description
Lyophilized NGS Library Preparation Kit, MDX219, consists of an end repair/dA-tailing mix module that combines the enzymes and buffers required for end-repair and dA-tailing to convert fragmented DNA into 5ʹ-phosphorylated and 3ʹ-dA-tailed DNA fragments, a ligase and buffer, enabling direct ligation of sequencing adapters and a high-fidelity PCR dried master mix for library amplification. The kit is designed to be straight forward and to demonstrate that the lyophilized kit can produce libraries equivalent to those achieved with traditional liquid formats.
Lyophilized NGS Library Preparation Kit (PCR-Free), MDX219-A
Lyophilized components for end-repair and A-tailing, ligation, and optionally library amplification. These can be stored at ambient temperature and are ready to use, just add DNA solution.
Documents & Resources
Lyophilized NGS Library Preparation Kit, MDX219-B
Lyophilized components for end-repair and A-tailing, ligation, and optionally library amplification. These can be stored at ambient temperature and are ready to use, just add DNA solution.
Documents & Resources
Catalogs & Brochures
Reagent Solutions for Molecular DiagnosticsNext-Generation Sequencing (NGS)Catalog
Glycerol-Free T4 DNA Ligase (HC)Glycerol-Free T4 DNA Ligase (HC)Catalog
High-Specificity Pfu HS MixHigh-Specificity Pfu HSCatalog
Related Products
Next Generation Sequencing
Get In Touch With A Specialist
Have questions about a product? Want to learn more about Meridian’s molecular or immunoassay reagent portfolio? We want to hear from you!