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Blockers

Immunoassay blockers are used in diagnostic assays to reduce non-specific binding and other interference that can lead to false-positive results. Specifically, antibody interference from human anti-mouse antibodies (HAMA), rheumatoid factor (RH), and heterophilic antibodies (HA) are a major concern in both paired monoclonal sandwich assays and competitive assays. In IgM detection assays, IgG antibodies which are present in 10-15x higher concentrations than IgM can also reduce assay sensitivity due to their sheer abundance. Specialized blockers are required to reduce antibody interference from HAMA, HA, RF and IgG to ensure an assay's accuracy and improve its sensitivity.

blockers

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Name
Type
Format
Host/Source
Isotype
Tested Apps
Unit
Catalog
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COA
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TRU Block, Heterophilic Antibody Interference, Active Blocker
Purified
Confidential
N/A
Blo
GM
A66800H
TRU Block 2, Heterophilic Antibody Interference, Active Blocker
Purified
Confidential
N/A
Blo
GM
A66802H
TRU Block 3, Heterophilic Antibody Interference, Active Blocker
Purified
Confidential
N/A
Blo
GM
A66803H
IgG, Mouse-Protein A (9-13 mg/mL) Dialyzed Azide Free
Purified
Mouse Serum
N/A
Blo
GM
A66186M-NA
IgG, Mouse-Caprylic Acid Cut
Purified
Mouse Serum
N/A
Blo
GM
A66187M
IgG, Mouse-Protein A (45-55 mg/mL)
Purified
Mouse Serum
N/A
Blo
GM
A66189M
IgG, Mouse Protein A (22-28 mg/mL)
Purified
Mouse Serum
N/A
Blo
GM
A66181M
IgG, Mouse-Protein A (50-55 mg/mL)
Purified
Mouse Serum
(blank)
Blo
GM
A66185M
IgG, Mouse-Protein A (9-13 mg/mL)
Purified
Mouse Serum
N/A
Blo
GM
A66186M
BSA, Standard Powder
Purified
Bovine
N/A
WB
GM
A64801B
TRU Block Ultra
Purified
Confidential
N/A
Blo,EIA,LF
GM
8000
TRU Block Ready
Aff.Pur.
Confidential
N/A
EIA,LF,Blo
ML
8001
TRU Block Ultra, No Azide
Purified
-
N/A
N/A
GM
8002
Elisa IgM Assay Diluent I
Purified
Goat
N/A
EIA
ML
8120
Goat anti Human IgG (H&L)
Aff.Pur.
Goat
N/A
N/A
MG
W99332G
Goat anti Human IgG+IgM+IgA Hl
HRP
Goat
N/A
IHC,WB
ML
W99591G
Goat anti Human IgG (Fc)
Alk.Phos.
Goat
N/A
EIA,WB
ML
W99008A
Goat anti Human IgG (Fc)
HRP
Goat
N/A
EIA,IHC,WB
ML
W99036P
Goat anti-human IgG(Fc)
Aff.Pur.
Goat
N/A
N/A
MG
W66040G
Goat anti Human IgG (H&L)
Alexa Fluor 488
Goat
N/A
EIA,IEP
MG
W01260AF
Goat anti-human IgG (h+l)
FITC
Goat
N/A
EIA,IEP
MG
W01262FL
Goat anti Human IgG (Fc)
Aff.Pur.
Goat
N/A
EIA
MG
W01252G
Goat anti-human IgG (Fc)
Purified
Goat
N/A
IEP
ML
L62540G
Goat anti-human IgG (Fc)
Monospecific
Goat
N/A
EIA,IEP,Nep,TIA
ML
L15406G
GOAT ANTI-HUMAN IGG (FC)-DILUT
Purified
Goat
N/A
Nep,TIA,IgM
ML
L15408G
Blocking Buffer For Lateral Fl
Liquid
-
N/A
LF
ML
J82300B
Coating Stabilizer & Block Buf
Liquid
-
N/A
N/A
ML
J16430D
Blocking Buffer For Elisa
N/A
-
N/A
EIA,WB
ML
J82100B
Goat anti Human IgG (Fc)
Aff.Pur.
Goat
N/A
EIA
MG
G5G16-048
Goat anti Human IgG (Fc)
HRP
Goat
N/A
EIA
ML
G5G16-0482

TRU Block – Targeting all Heterophile Interferences

TRUE Positive Result

tru block range

Meridian's Blockers Range

  • Designed to remove human-anti-mouse interference (HAMA), heterophilic interference (HA), and rheumatoid factor (RF) interference.
  • Suitable for double mouse monoclonal and competitive immunoassays.
  • Effective at low concentrations (100x-1,000x less than passive blocking agents such as animal serums).

tru block performance data

tru block animal igg range

IgG Blockers for IgM Detection Assays

Competition between IgG and IgM antibodies for antigen binding sites can significantly reduce assay sensitivity in IgM detection assays. In addition, IgG can form immune complexes with Rheumatoid Factor (RF) and compete with specific IgM for substrate binding sites. Removal of IgG and RF-IgM can be accomplished by pre-treating the patient specimen with goat anti-human IgG or IgM diluent.

tru block reduce-igg-competition

FAQs: Immunoassay Interference Blockers

Immunoassays used for human in vitro diagnostics (IVD) typically use animal-derived antibodies to recognize specific disease markers. Some patients have antibodies in their blood that can react with animal antibodies in the immunoassay and cause a false result. Although the frequency of these interferences is low, false-positive results have a significant negative impact on the quality and competitiveness of a diagnostic assay as well as on the lives of those individuals who have been falsely diagnosed.

Human anti-mouse antibodies (HAMA) is the common antibody interference which causes both positive and negative interferences in two-site mouse monoclonal antibody-based assays.

No. HAMA only represents one type of heterophilic antibody (HA) interference – others include HA to animals such as goat (HAGA), sheep (HASA), and rabbit (HARA) which can cause false results when antibodies originating from these animals are used in immunoassays. In addition to HA there is another class of interference called Rheumatoid factor (RF), which is an autoantibody that reacts with the patient’s own immunoglobulin (Ig) and can cross react with animal Ig, similar to HA/HAMA
interference.

Rheumatoid factor is an autoantibody (usually IgM) directed against the Fc portion of IgG. It is common in people with rheumatoid factor, occurring in about half of all people with the disease, but about 1 in 20 people without rheumatoid arthritis can also have high levels and is more common in elderly patients.

RF, similar to heterophilic antibodies, has the potential to cause falsely elevated test results by cross-linking the assay antibodies, even in the absence of analyte, most often via binding to the Fc-part of assay antibodies. RF can react against different species of IgG, including human and rabbit.

Our recommendations are: a) use the species of the blocker the same as the host of the capture or detection antibody; 2) use at a concentration of Mouse or Goat IgG equivalent to ten times the concentration of the MAb/PAb being used in the assay. (e.g. if 5ug/mL of Ab/conjugate, add 50ug/mL of animal IgG)

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Have questions about a product? Want to learn more about Meridian’s molecular or immunoassay reagent portfolio? We want to hear from you!

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