A BSA blocking buffer is a solution used in laboratory experiments, particularly in immunoassays like ELISA and western blot. It is used to reduce background noise and non-specific binding in the assay by coating the surface of the plate or membrane with a protein, such as Bovine Serum Albumin (BSA), that will compete with the assay's target protein for binding sites. By using a BSA blocking buffer, it will help to minimize non-specific binding of primary and secondary antibodies, and improve the specificity and sensitivity of the assay. This buffer is commonly used in research and diagnostic applications.