Glycerol-Free Taq HS 50U/µL

Glycerol-Free Taq HS 50U/µL has been optimized to deliver excellent, stable performance without glycerol, meaning it is also compatible with lyophilization, as a stand-alone enzyme, or when combined with additional assay components in a reaction mix.


Request a Sample

Have questions about a product?

Contact us to learn more about Meridian’s molecular or immunoassay reagent portfolio. We want to hear from you!

Contact A Specialist

Meridian's Glycerol-Free Taq HS DNA Polymerase

  • High concentration of Taq DNA Polymerase allowing for concentrated bulk production of PCR master mixes.

  • Retains the same reproducible performance as with conventional formats.

  • Separate Taq, antibody and enzyme dilution buffer, for greater assay development flexibility.

  • Glycerol-free solution, ideal for preparation of dried-down PCR tests.

Efficient and sensitive amplification with both wet (black) and lyophilized (blue) PCR tests

Glycerol-Free Taq HS DNA Polymerase graph
A 10-fold serial dilution of template DNA was used to set up two sets of qPCR assays. One set was lyophilized (blue) and the other left as a wet mix (black). Results illustrate that lyophilization of Glycerol-Free Taq HS does not have an effect on the quality of the qPCR, as both the assays demonstrate identical efficiency and sensitivity.

Glycerol-Free Taq HS 50U/µL, MDX011

Available in 0.02 mL (1,000 Units) or 0.5 mL (25,000 Units) aliquots

Description

Glycerol-Free Taq HS 50U/µL is supplied as a separate 10x, high‑performance glycerol‑free Taq DNA Polymerase, glycerol‑free Taq antibody, and glycerol‑free enzyme dilution buffer. Separating these components allows greater flexibility in assay development, making Glycerol-Free Taq HS 50 U/µL suitable for difficult, multiplex PCR that require fast amplification, whilst maintaining both high specificity and sensitivity.

Glycerol-Free Taq HS 50U/µL is ideal for PCR kit manufactures and PCR molecular test facilities, requiring large amounts of DNA polymerases. The highly concentrated Glycerol-Free Taq HS 50U/µL allows for concentrated bulk production of PCR master mixes and the glycerol free format is useful when using automated pipetting robots and other applications where accurate pipetting of small volumes is crucial.


Specifications

Description A lyophilization-compatible Taq DNA polymerase, separate Taq antibody and enzyme dilution buffer, suitable for difficult, multiplex molecular tests that require fast amplification, whilst maintaining both high specificity and sensitivity.
Concentration Between 50 and 60 U/µL (see CoA)
Purity >50 % (as a percentage of total protein)
Glycerol content <0.02 %
Appearance Clear, colorless solution
Hot Start Antibody mediated
Application PCR, real-time PCR, two-step RT-qPCR
Sample type cDNA, DNA
Presentation 3 vials
Storage -20 °C
Mix stability See outer label
Consistency ±0.5 Ct variance between test and reference sample
DNA Contamination None detected in PCR amplification with traces overlay with the negative control on E. coli and mouse genomic DNA specific targets.
DNase Contamination No detectable degradation

FAQs: Glycerol-Free Taq HS

Glycerol-Free Taq HS 50U/µL is supplied as a separate 50 U/μL, high‑performance glycerol‑free Taq DNA polymerase, glycerol‑free Taq antibody and glycerol‑free enzyme dilution buffer. Separating these components allows greater flexibility in developing PCR tests.

Glycerol-Free Taq HS 50 U/µL is ideal for PCR kit manufactures and DNA testing facilities, requiring large amounts of DNA polymerases. The highly concentrated Glycerol-Free Taq HS 50 U/µL allows for concentrated bulk production of PCR master mixes.
Concentrated bulks in glycerol-free formats are also attractive when using automated pipetting robots and other applications where accurate pipetting of small volumes is crucial, as glycerol makes the solution more viscous.
Concentrated PCR master mixes are easier to lyophilize due to the reduced volume of the concentrated mix.

Glycerol is a cryoprotectant and is normally part of the storage buffer, where it serves to protect Taq DNA polymerase during freezing conditions. The high concentration of Glycerol-Free Taq HS 50 U/µL means that you get the same protection to freeze/thawing as a DNA polymerase that contains glycerol.

A lyophilized PCR molecular test will remain stable for up to 2 years at ambient temperature if correctly stored in sealed pouches.

Yes, Glycerol-Free Taq HS 50 U/µL is ideal for any multiplex PCR assays, particularly quantitative PCR molecular tests that require fast amplification, whilst maintaining both high specificity and sensitivity.

Get In Touch With A Specialist

Have questions about a product? Want to learn more about Meridian’s molecular or immunoassay reagent portfolio? We want to hear from you!


By submitting your information in this form, you agree that your personal information may be stored and processed in any country where we have facilities or service providers, and by using our “Contact Us” page you agree to the transfer of information to countries outside of your country of residence, including to the United States, which may provide for different data protection rules than in your country. The information you submit will be governed by our Privacy Statement.

Learn More About Us

Learn More
Welcome to
logo blue

Please select your country to continue

Continue
CN