Air-Dryable™ LAMP/RT-LAMP Mixes
Meridian’s Air-Dryable™ DNA LAMP and RNA/DNA LAMP Mixes are ready-to-use, 4x concentrated, fully optimized mixes for isothermal amplification methods such as LAMP, designed to dramatically speed up assay development time and reduce TTR by 50%.
Isothermal amplification is a highly sensitive nucleic acid amplification method that has gained popularity in recent years due to its advantages over qPCR, such as quicker time to results, simple assay workflow, and point-of-care usability. However, the initial assay design and development can be more complex with 4 to 6 primers which are used in combination to generate a robust, fast, and sensitive result. Through extensive R&D research, Meridian has discovered a blend of enzymes, dNTPs, Mg2+, excipients, and stabilizers that are used with customer-selected primers and probes for creating any custom DNA or RNA LAMP assay. Experimental data on both RNA and DNA templates demonstrate that the mixes are up to 2x faster than other suppliers, generating results in half the time. In addition, the mixes incorporate Meridian’s new Air-Dryable™ technology and can be used in a liquid format or oven-dried to create an ambient-temperature stable assay (please see the product guide and FAQs for recommendations on oven drying parameters).
By using a ready-to-use master mix such as Air-Dryable™ DNA LAMP or RNA/DNA LAMP, assay development is faster, simpler, and more efficient, speeding up your product development time and allowing you to commercialize your new products faster.
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Results in half the time - Fastest reaction kinetics on the market.
Air-Dryable™ RNA/DNA LAMP mix was air-dried with Dengue type 2 primers at 50°C for 80 mins. The sensitivity of air-dried Air-Dryable™ RNA/DNA LAMP mix (red) was compared to its liquid (blue) format and NEB WarmStart® (orange) in a serial dilution of the target RNA (Dengue type 2 crude lysate). Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence. Error bars represent standard deviation over 4 technical replicates. The results illustrate that the speed of the Air-Dryable™ RNA/DNA LAMP mix is twice as fast as a NEB WarmStart® in either the dry or liquid format.
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