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DNA sample preparation for next-generation sequencing (NGS) involves several steps. Firstly a DNA library is created that is suitable for the specific NGS platform to be used. This may have a target enrichment step prior to the addition of adaptors and amplification, with the quality of the library being depend on the nature of the DNA sample, input volume and level of difficulty (samples with high AC- or GC-rich regions). Each step having the potential to introduce errors or biases that could significantly impact library quality. Meridian offers molecular reagents for library preparation that help reduce errors and bias to a minimum whilst allowing for efficiency and sensitivity.
| Name | Details | Application | Catalog | Order a Sample | Sizes | Description | Wet(Liquid) | Air-Dryable | Name | Glycerol-Free | Lyo-Ready | Concentration | High Concentration | Detail | Safety Data Sheet | COA/Test Release | User Guide | Product Handling Guide | Product Manual | ||
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| Gycerol-free T4 DNA Ligase (HC) | DNA | NGS | MDX200 | Order a Sample | Available in 1,000 Unit or 10,000 Unit aliquots | Glycerol-free, high concentration enzyme that efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. Ideal for lyophilization. | Yes | Yes | Requires excipients | Yes | Yes | 50 Weiss unit/µL | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide | ||||
| Glycerol-Free High-Fidelity Pfu (HC) | DNA | PCR | MDX203 | Order a Sample | Available in 0.025 mL (500 Units) or 1 mL (20,000 Units) aliquots | Glycerol-free, high concentration, high fidelity DNA polymerase with separate dilution and reaction buffer, ideal for NGS library amplification and gene expression. Ideal for lyophilization. | Yes | Yes | Requires excipients | Yes | Yes | 20 U/µL | Yes | View Details | Certifate of Analysis | Product Handling Guide | |||||
| Glycerol-Free T4 Polynucleotide Kinase (HC) | DNA | NGS | MDX206 | Order a Sample | Available in 25 µL (500 Units) or 250 µL (5,000 Units) aliquots | Glycerol-free enzyme used to phosphorylate the ends of both DNA and RNA during labeling and ligation, mainly used in next-generation sequencing (NGS) library preparation. Ideal for lyophilization. | Yes | Yes | Requires excipients | Yes | Yes | 20 U/µL | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide | ||||
| Glycerol-Free T4 DNA Polymerase (HC) | DNA | NGS | MDX207 | Order a Sample | Available in 50 µL (500 Units) or 500 µL (5,000 Units) aliquots | Glycerol-free, high concentration DNA polymerase used for generating blunt ends on any duplex DNA molecule, used in next-generation sequencing (NGS) library preparation. Ideal for lyophilization. | Yes | Yes | Requires excipients | Yes | Yes | 20 U/µL | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide | ||||
| Glycerol-Free DNA Pol I Klenow Fragment (HC) | DNA | NGS | MDX208 | Order a Sample | Available in 200 µL (10,000 Units) or 1 mL (50,000 Units) aliquots | Glycerol-free, high concentration DNA polymerase used for generating blunt ends on any duplex DNA molecule, used in next-generation sequencing (NGS) library preparation. Ideal for lyophilization. | Yes | Yes | Requires excipients | Yes | Yes | 50 U/µL | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide | ||||
| Glycerol-Free DNA Pol I Klenow Fragment exo- (HC) | DNA | NGS | MDX218 | Order a Sample | Available in 50 µL (10,000 Units) or 1 mL (50,000 Units) aliquots | Glycerol-free, high concentration DNA Pol I Klenow Fragment exo-, designed for dA- and dT-tailing for next-generation sequencing (NGS) adapter ligation and DNA sequencing by the Sanger method. | Yes | Yes | Requires excipients | Yes | Yes | 20 U/µL | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide | ||||
| Lyophilized NGS Library Preparation Kit (PCR-Free) | DNA | NGS | MDX219-A | Order a Sample | Available as 8 reactions, with and without PCR amplification | Lyophilized components for end-repair and A-tailing, ligation, and optionally library amplification. These can be stored at ambient temperature and are ready to use, just add DNA solution. | Yes | Lyo-Ready | Lyo-Ready | Yes | Requires excipients | N/A | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Manual | ||||
| Lyophilized NGS Library Preparation Kit | DNA | NGS | MDX219-B | Order a Sample | Available as 8 reactions, with and without PCR amplification | Lyophilized components for end-repair and A-tailing, ligation, and optionally library amplification. These can be stored at ambient temperature and are ready to use, just add DNA solution. | Yes | Yes | Lyo-Ready | Yes | Yes | N/A | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Manual |
Meridian offers diagnostic manufacturers the complete solution with over 3,000 antigens and antibodies covering more than 500 diseases and an extensive range of specialty qPCR/RT-qPCR master mixes and molecular enzymes all manufactured under ISO13485 regulations.
Large Sample Size
Lot Reservation
ISO13485
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