Lyophilized NGS Enzymatic DNA Fragmentation Kit
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Lyophilized NGS Enzymatic DNA Fragmentation Kit
- Ambient Shipping & Storage (No Cold-Chain) – Lyophilized, glycerol-free; ship and store at 15-25 °C
- Precise Time-Controlled Enzymatic Fragmentation – Reproducible fragment sizes without mechanical shearing
- Simplified Workflow – Fewer steps, reduced variability, no sonication
- Easy Assay Integration – Ready-to-rehydrate format at point-of-use; just add DNA
- Scalable for Development & Throughput – Supports low-, medium- and high-throughput workflows
- Suitable with automated workflows
WGS Workflow
The straightforward design ensures that the NGS library preparation process is fast and convenient, contributing to cost-effective, sustainable, and accessible NGS technology.
Lyophilized NGS Enzymatic DNA Fragmentation Kit, MDX224
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Description
By replacing mechanical shearing with controlled enzymatic fragmentation, Meridian’s Lyophilized NGS Enzymatic DNA Fragmentation Kit (MDX224) simplifies NGS library preparation while improving reproducibility and operational flexibility. The fully lyophilized, glycerol-free format eliminates cold-chain requirements and reduces workflow complexity, enabling more consistent performance across sites and scales. Compatible with established short-read library preparation workflows, the kit is designed to support integration into NGS assay development and kit formats.
Key Product Benefits
- Eliminates Cold-Chain Dependence
Ambient storage and global shipping simplify logistics and deployment. - Improves Workflow Consistency
Fully lyophilized, glycerol-free formulation reduces variability versus frozen enzymes. - Delivers Predictable Fragmentation
Precise time-controlled enzymatic fragmentation without mechanical shearing. - Reduces Hands-On Time and Complexity
Fewer steps and no specialized instrumentation required. - Ready-to-rehydrate at point of use
Simplifies assay execution for end users while reduces reagent handling. - Scales with Development and Throughput
Suitable for low-, medium-, and high-throughput NGS applications. - Built for Kit and Assay Developers
Designed to support integration into customer-defined NGS workflows.
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FAQs: Lyophilized NGS Enzymatic DNA Fragmentation Kit
What is enzymatic DNA fragmentation?
Enzymatic DNA fragmentation uses nuclease-based reactions to randomly cleave DNA into fragments suitable for next-generation sequencing (NGS) library preparation, without the need for mechanical shearing.
How does enzymatic fragmentation compare to mechanical shearing (e.g. sonication)?
Enzymatic fragmentation is faster, typically requires less input DNA and avoids specialized equipment. It also enables tighter workflow integration and improved reproducibility, particularly for low-input or high-throughput applications.
What fragment sizes can be generated with this kit?
Fragment size is controlled by reaction time, temperature, and enzyme concentration. Typical ranges span approximately 150 bp to 800 bp, depending on sample type and input DNA concentration.
How reproducible is enzymatic fragmentation?
When optimized, enzymatic fragmentation provides highly reproducible fragment size distributions across runs and operators, making it well suited for automation and standardized workflows.
Can enzymatic fragmentation be used for all NGS library types?
The kit is compatible with most standard NGS workflows, including whole-genome sequencing (WGS), targeted sequencing, amplicon-based enrichment and microbial sequencing.
How long does the fragmentation reaction take?
Typical reactions take 10–25 minutes, depending on desired fragment size and protocol design.
Is the kit suitable for automation?
Yes. The lyophilized format, reduced pipetting steps, and consistent reaction conditions make the kit highly compatible with liquid handling automation.
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