DNA-RNA Extraction Controls
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Products (6)
| Name | Details | Application | Catalog | Order a Sample | Sizes | Description | Wet(Liquid) | Air-Dryable | Name | Glycerol-Free | Lyo-Ready | Concentration | High Concentration | Detail | Safety Data Sheet | COA/Test Release | User Guide | Product Handling Guide | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| VLP-RNA Extraction Control Red | VLP Extraction Controls | RT-qPCR | MDX068 | Order a Sample | Available in 1 mL (~1?10^4 copies/L) and 20 mL (~1?10^4 copies/L) aliquots | An Internal Control RNA sequence, with no known homology to any organism, encapsidated in a virus-like particle and a VLP Detection Mix containing primers and probe to the internal control RNA sequence. |
Yes | Requires excipients | Air-Dryable,Lyo-Ready | Yes | Requires excipients | 10^4 copies/L | No | View Details | Safety Data Sheet | COA/Test Release | ||||
| VLP-RNA Extraction Control Orange | VLP Extraction Controls | RT-qPCR | MDX069 | Order a Sample | Available in 1 mL (~1?10^4 copies/L) and 20 mL (~1?10^4 copies/L) aliquots | An Internal Control RNA sequence, with no known homology to any organism, encapsidated in a virus-like particle and a VLP Detection Mix containing primers and probe to the internal control RNA sequence. |
Yes | Requires excipients | Air-Dryable,Lyo-Ready | Yes | Requires excipients | 10^4 copies/L | No | View Details | Safety Data Sheet | COA/Test Release | ||||
| qPCR Extraction Control Red | Extraction Controls | qPCR | MDX026 | Order a Sample | Available in 10 mL (2,000 Rxns) aliquots | Control used to monitor the success of a qPCR assay from lysis/extraction to amplification and reduces the chance of obtaining false negative results from the sample DNA. |
Yes | No | No | No | No | 25x | No | View Details | Safety Data Sheet | COA/Test Release | Product Handling Guide | |||
| qPCR Extraction Control Orange | Extraction Controls | qPCR | MDX027 | Order a Sample | Available in 10 mL (2,000 Rxns) aliquots | Control used to monitor the success of a qPCR assay from lysis/extraction to amplification and reduces the chance of obtaining false negative results from the sample DNA. |
Yes | No | No | No | No | 25x | No | View Details | Safety Data Sheet | COA/Test Release | Product Handling Guide | |||
| RT-qPCR Extraction Control Red | Extraction Controls | RT-qPCR | MDX028 | Order a Sample | Available in 1 mL (500 Rxns) aliquots | Control used to monitor the success of an RT-qPCR assay from lysis/extraction to amplification and reduces the chance of obtaining false negative results from the sample RNA. |
Yes | No | No | No | No | 25x | No | View Details | Safety Data Sheet | COA/Test Release | Product Handling Guide | |||
| RT-qPCR Extraction Control Orange | Extraction Controls | RT-qPCR | MDX029 | Order a Sample | Available in 1 mL (500 Rxns) aliquots | Control used to monitor the success of an RT-qPCR assay from lysis/extraction to amplification and reduces the chance of obtaining false negative results from the sample RNA. |
Yes | No | No | No | No | 25x | No | View Details | Safety Data Sheet | COA/Test Release | Product Handling Guide |
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- Extraction control undergoes the same processing as test sample.
- Confirms successes of extraction step and monitors co-purification of PCR inhibitors.
- Probe based designed specifically for multiplex qPCR/RT-qPCR assays.
The extraction controls comprise an internal control sequence in use E. coli cells or artificial micelles and a control mix (containing specific primer and probe (emission wavelength = 560nm or 670nm)) for qPCR detection. The cells are added to the sample or lysis buffer and lysed and extracted with the sample for downstream amplification. The detection of extraction control acts as a PCR positive control and confirms the success of the extraction and amplification steps, avoiding the misinterpretation of a PCR false negative result.
FAQ's
The benefit of the artificial Extraction Control in evaluation of the extraction process is the possibility to validate sample extraction process. Signal derived from the Extraction Control confirms the success of the extraction step and monitors co-purification of PCR inhibitors that may cause biased or PCR false negative results. Housekeeping genes or spike in controls do not monitor the whole extraction process, especially the lysis step and in the case of housekeeping genes, extraction efficiency and degraded samples as well as the possibility of the housekeeping gene expression level changing.
The primers and probe for the Extraction Controls are in the Control Mix.
The two colors are for the fluorescent dyes on the Extraction Control probe. Control Mix Red (Cy5 – emission wavelength = 670nm) and Control Mix Orange (HEX – emission wavelength = 560nm). This allows you to choose one that will fit with your existing protocol in a multiplex assay.
No, the Extraction Control will work with commercially available silica-membrane extraction kits and CHELEX matrices and has been tested on a wide range of qPCR platforms.
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