Lyo-Ready & Air-Dryable Master Mixes & Reagents

Molecular diagnostic tests are progressively moving towards lyophilized and air-dried formats. There are several advantages for this, including room temperature shipping and storage, extended shelf-life, and increased flexibility in sample volume. However, to be compatible with drying, enzyme preparations must be glycerol-free and include specialized excipients that preserve the mixture as it is exposed to various conditions, including freezing, temperature ramps, vacuum, and dehydration.

Products (75)

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Name	Details	Application	Catalog	Order a Sample	Sizes	Description	Wet(Liquid)	Air-Dryable	Name	Glycerol-Free	Lyo-Ready	Concentration	High Concentration	Detail	Safety Data Sheet	COA/Test Release	User Guide	Product Handling Guide
Lyo-Ready AOF dUTP RNA/DNA qPCR Urine	Urine, RNA	RT-qPCR	MDX353	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	An optimized RT-qPCR master mix tolerant to inhibitors in urine. Perfect for multiplexing, high throughput assays where there is a greater risk of false positives from cross-over contamination.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready AOF dUTP DNA qPCR Urine	DNA	qPCR	MDX352	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	An optimized qPCR master mix tolerant to inhibitors in urine. Perfect for multiplexing, high throughput assays where there is a greater risk of false positives from cross-over contamination.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready AOF dUTP RNA/DNA qPCR Saliva	Saliva, Sputum, RNA	RT-qPCR	MDX333	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	An optimized RT-qPCR master mix tolerant to inhibitors in saliva and sputum. Perfect for multiplexing, high throughput assays where there is a greater risk of false positives from cross-over contamination.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready AOF dUTP DNA qPCR Saliva	Saliva, Sputum, DNA	qPCR	MDX332	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	An optimized qPCR master mix tolerant to inhibitors in saliva and sputum. Perfect for multiplexing, high throughput assays where there is a greater risk of false positives from cross-over contamination.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready AOF dUTP RNA/DNA qPCR Blood	Blood, Plasma, Serum, RNA	RT-qPCR	MDX323	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	An optimized RT-qPCR master mix tolerant to inhibitors in whole blood and plasma and compatible with all anticoagulants. Perfect for multiplexing, high throughput assays where there is a greater risk of false positives from cross-over contamination.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready AOF dUTP DNA qPCR Blood	Blood, Plasma, Serum, DNA	qPCR	MDX322	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	An optimized qPCR master mix tolerant to inhibitors in whole blood and plasma and compatible with all anticoagulants. Perfect for multiplexing, high throughput assays where there is a greater risk of false positives from cross-over contamination.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Glycerol-Free Thermostable DNA Helicase (HC)	DNA	LAMP, RPA, RCA, SDA	MDX242	Order a Sample	Available in 20 µL (1 µg) or 200 µL (10 µg) aliquots	Glycerol-free, high concentration enzyme used to unwind double-stranded DNA. It operates at up to 65°C, ideal for demanding diagnostic workflows such as NGS library preparation, SNP detection and mutation analysis	Yes	Yes	Requires excipients	Yes	Yes	50 µg/mL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free Phi29 DNA Polymerase (HC)	DNA	RCA, WGA	MDX240	Order a Sample	Available in 20 µL (2,000 Unit) or 100 µL (10,000 Unit) aliquots	Glycerol-free, high concentration enzyme with exceptional strand displacement and processive synthesis properties, ideal for rolling-circle amplification (RCA).	Yes	Yes	Requires excipients	Yes	Yes	100 U/?L	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Lyo-Ready Inhibitor-Tolerant qPCR Mix	Blood, saliva, urine, stool, tissue, DNA	qPCR	MDX184	Order a Sample	Available in 1,000 Unit or 10,000 Unit aliquots	Universal, high concentration, inhibitor tolerant qPCR master mix, for direct amplification from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood, sputum, saliva and stool.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Lyo-Ready Direct DNA LAMP Urine, 4x	Urine, DNA	LAMP	MDX154	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready Direct RNA/DNA LAMP Urine, 4x	Urine, RNA	RT-LAMP	MDX155	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready Genotyping Direct qPCR Urine, 4x	Urine, DNA	SNP Genotyping	MDX158	Order a Sample	Available in 1,000 Unit or 10,000 Unit aliquots	Inhibitor tolerance to 20% urine. Compatible with all dual-label probe chemistries for detection of genetic variants, such as single nucleotide and copy number variants, to give highly reproducible allelic discrimination.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Lyo-Ready Genotyping Direct qPCR FFPE, 4x	FFPE, DNA	SNP Genotyping	MDX168	Order a Sample	Available in 1,000 Unit or 10,000 Unit aliquots	Better extraction buffers and greater tolerance to crude FFPE samples. Compatible with all dual-label probe chemistries for detection of genetic variants, such as single nucleotide and copy number variants, to give highly reproducible allelic discrimination.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details		Certifate of Analysis	User Guide	Product Handling Guide
Glycerol-Free Taq HS High Conc. DNA Polymerase	DNA	PCR, NGS	MDX170	Order a Sample	Available in 0.025 mL (1,000 Units) or 0.5 mL (25,000 Units) aliquots	Glycerol-free, high concentration, high fidelity DNA polymerase with separate dilution and reaction buffer, ideal for NGS library amplification and gene expression. Ideal for lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	50 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Lyo-Ready Inhibitor-Tolerant RT-qPCR Mix	Blood, saliva, urine, stool, tissue, RNA	RT-qPCR	MDX185	Order a Sample	Available in 1,000 Unit or 10,000 Unit aliquots	Universal, high concentration, inhibitor tolerant RT-qPCR master mix, for direct amplification from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood, sputum, saliva and stool.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Gycerol-free T4 DNA Ligase (HC)	DNA	NGS	MDX200	Order a Sample	Available in 1,000 Unit or 10,000 Unit aliquots	Glycerol-free, high concentration enzyme that efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. Ideal for lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	50 Weiss unit/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free RNase H (HC)	RNA	RT-qPCR	MDX202	Order a Sample	Available in 100 µL (2,500 Unit) or 250 µL (12,500 Unit) aliquots	Glycerol-free, high concentration RNA-specific endonuclease that hydrolyzes the phosphodiester bonds of RNA, when hybridized to DNA. Ideal for lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	50 U/µL	Yes	View Details		Certifate of Analysis		Product Handling Guide
Glycerol-Free High-Fidelity Pfu (HC)	DNA	PCR	MDX203	Order a Sample	Available in 0.025 mL (500 Units) or 1 mL (20,000 Units) aliquots	Glycerol-free, high concentration, high fidelity DNA polymerase with separate dilution and reaction buffer, ideal for NGS library amplification and gene expression. Ideal for lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	20 U/µL	Yes	View Details		Certifate of Analysis		Product Handling Guide
Glycerol-Free T7 RNA Polymerase (HC)	RNA	NASBA	MDX201	Order a Sample	Available in 50,000 Unit or 500,000 Unit aliquots	Glycerol-free, high concentration DNA-dependant RNA Polymerase that requires a T7 promoter sequence, it an ideal enzyme for in-vitro synthesis of labeled or unlabeled RNA for research, diagnostic or clinical applications. Ideal for lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	1,000 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free HS Tth DNA Polymerase (HC)	DNA, RNA	RT-qPCR	MDX205	Order a Sample	Available in 50 µL (4,000 Unit) or 500 µL (100,000 Unit) aliquots	Glycerol-free, high concentration, thermostable DNA polymerase with intrinsic reverse transcriptase (RT) activity and unlike other RT enzymes, it is not associated with RNase H activity. Ideal for lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	200 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free T4 Polynucleotide Kinase (HC)	DNA	NGS	MDX206	Order a Sample	Available in 25 µL (500 Units) or 250 µL (5,000 Units) aliquots	Glycerol-free enzyme used to phosphorylate the ends of both DNA and RNA during labeling and ligation, mainly used in next-generation sequencing (NGS) library preparation. Ideal for lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	20 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free T4 DNA Polymerase (HC)	DNA	NGS	MDX207	Order a Sample	Available in 50 µL (500 Units) or 500 µL (5,000 Units) aliquots	Glycerol-free, high concentration DNA polymerase used for generating blunt ends on any duplex DNA molecule, used in next-generation sequencing (NGS) library preparation. Ideal for lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	20 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free DNA Pol I Klenow Fragment (HC)	DNA	NGS	MDX208	Order a Sample	Available in 200 µL (10,000 Units) or 1 mL (50,000 Units) aliquots	Glycerol-free, high concentration DNA polymerase used for generating blunt ends on any duplex DNA molecule, used in next-generation sequencing (NGS) library preparation. Ideal for lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	50 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free DNA Pol I Klenow Fragment exo- (HC)	DNA	NGS	MDX218	Order a Sample	Available in 50 µL (10,000 Units) or 1 mL (50,000 Units) aliquots	Glycerol-free, high concentration DNA Pol I Klenow Fragment exo-, designed for dA- and dT-tailing for next-generation sequencing (NGS) adapter ligation and DNA sequencing by the Sanger method.	Yes	Yes	Requires excipients	Yes	Yes	20 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Lyo-Ready Direct DNA LAMP Saliva, 4x	Saliva, Sputum, DNA	LAMP	MDX134	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready Direct RNA/DNA LAMP Saliva, 4x	Saliva, Sputum, RNA	RT-LAMP	MDX135	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Air-Dryable Direct DNA qPCR Stool, 4x	Stool, DNA	qPCR	MDX140	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 20% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.	Yes	Yes	Air-Dryable	Yes	No	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Air-Dryable Direct RNA/DNA qPCR Stool, 4x	Stool, RNA	RT-qPCR	MDX141	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 5% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.	Yes	Yes	Air-Dryable	Yes	No	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready Direct DNA qPCR Stool, 4x	Stool, DNA	qPCR	MDX142	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 20% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready Direct RNA/DNA qPCR Stool, 4x	Stool, RNA	RT-qPCR	MDX143	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 5% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready Direct DNA LAMP Stool, 4x	Stool, DNA	LAMP	MDX144	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 20% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready Direct RNA/DNA LAMP Stool, 4x	Stool, RNA	RT-LAMP	MDX145	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 5% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready Genotyping Direct qPCR Stool, 4x	Stool, DNA	SNP Genotyping	MDX148	Order a Sample	Available in 1,000 Unit or 10,000 Unit aliquots	Inhibitor tolerance to 20% stool. Compatible with all dual-label probe chemistries for detection of genetic variants, such as single nucleotide and copy number variants, to give highly reproducible allelic discrimination.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details		Certifate of Analysis		Product Handling Guide
Air-Dryable Direct DNA qPCR Urine, 4x	Urine, DNA	qPCR	MDX150	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 60% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.	Yes	Yes	Air-Dryable	Yes	No	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Air-Dryable Direct RNA/DNA qPCR Urine, 4x	Urine, RNA	RT-qPCR	MDX151	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.	Yes	Yes	Air-Dryable	Yes	No	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready Direct DNA qPCR Urine, 4x	Urine, DNA	qPCR	MDX152	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details		Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready Direct RNA/DNA qPCR Urine, 4x	Urine, RNA	RT-qPCR	MDX153	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details		Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready Direct RNA/DNA qPCR Saliva, 4x	Saliva, Sputum, RNA	RT-qPCR	MDX133	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready Direct DNA qPCR Saliva, 4x	Saliva, Sputum, DNA	qPCR	MDX132	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Air-Dryable Direct RNA/DNA qPCR Saliva, 4x	Saliva, Sputum, RNA	RT-qPCR	MDX131	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.	Yes	Yes	Air-Dryable	Yes	No	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Air-Dryable Direct DNA qPCR Saliva, 4x	Saliva, Sputum, DNA	qPCR	MDX130	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.	Yes	Yes	Air-Dryable	Yes	No	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Lyo-Ready Genotyping Direct qPCR Blood, 4x	Blood, Plasma, Serum, DNA	SNP Genotyping	MDX128	Order a Sample	Available in 1,000 Unit or 10,000 Unit aliquots	Inhibitor tolerance to 10% whole blood, 20% plasma. Compatible with all dual-label probe chemistries for detection of genetic variants, such as single nucleotide and copy number variants, to give highly reproducible allelic discrimination.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Air-Dryable Direct DNA LAMP Blood, 4x	Blood, Plasma, Serum, DNA	LAMP	MDX126	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.	Yes	Yes	Air-Dryable	Yes	No	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready Direct RNA/DNA LAMP Blood, 4x	Blood, Plasma, Serum, RNA	RT-LAMP	MDX125	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Air-Dryable 1-Step RT-qPCR Mix, 4x	RNA	RT-qPCR	MDX095	Order a Sample	Available in 5 mL (500 Rxns) or 50 mL (10,000 Rxns) aliquots	Glycerol-free RT-qPCR mix containing Taq polymerase, reverse transcriptase, reaction buffer, dNTPs, MgCl2 and air-dry compatible excipients, ideal for developing ambient temperature, multiplex molecular assays.	Yes	Yes	Air-Dryable	Yes	No	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Air-Dryable qPCR Mix, 4x	DNA	qPCR	MDX082	Order a Sample	Available in 5 mL (500 Rxns) or 50 mL (10,000 Rxns) aliquots	Glycerol-free qPCR mix containing Taq polymerase, reaction buffer, dNTPs, MgCl2 and air-dry compatible excipients, ideal for developing ambient temperature, multiplex molecular assays.	Yes	Yes	Air-Dryable	Yes	No	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide
Air-Dryable Direct DNA qPCR Blood, 4x	Blood, Plasma, Serum, DNA	qPCR	MDX092	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 20% whole blood, 20% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.	Yes	Yes	Air-Dryable	Yes	No	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready LAMP Mix, 4x	DNA	LAMP	MDX097	Order a Sample	Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots	Glycerol-free mix for isothermal applications such as LAMP, containing Bst DNA polymerase, reaction buffer, dNTPs and lyo-compatible excipients, ideal for developing ambient temperature, LAMP assays.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details		Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready RT-LAMP 1-Step Mix, 4x	RNA	RT-LAMP	MDX108	Order a Sample	Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots	Glycerol-free mix for isothermal applications such as RT-LAMP, containing Bst DNA polymerase, reverse transcriptase, reaction buffer, dNTPs and lyo-compatible excipients, ideal for developing ambient temperature, LAMP assays.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready dUTP 1-Step RT-qPCR Mix	RNA	RT-qPCR	MDX113	Order a Sample	Available in 10 mL (1,000 Rxns) or 100 mL (10,000 Rxns) aliquots	Glycerol-free mix containing Taq polymerase, RNase Inhibitor, reaction buffer, dNTP/dUTP mix, MgCl2 and lyo-excipients and separate reverse transcriptase , developed to remove background PCR product contamination.	Yes	Yes	Lyo-Ready	Yes	Yes	2x	Yes	View Details		Certifate of Analysis		Product Handling Guide
Air-Dryable Direct DNA qPCR Plant, 4x	Plant, DNA	qPCR	MDX116	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	No need for extraction, compatible with 25% Plant lysate (SDS, NaOH). Perfect for multiplexing and fast cycling conditions for in-field qPCR testing. Used for genotyping and transgene or pathogenic detection.	Yes	Yes	Air-Dryable	Yes	No	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Air-Dryable RT-LAMP 1-Step Mix, 4x	RNA	RT-LAMP	MDX118	Order a Sample	Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots	Glycerol-free mix for isothermal applications such as RT-LAMP, containing Bst DNA polymerase, reverse transcriptase, reaction buffer, dNTPs and air-dryable excipients, ideal for developing ambient temperature, LAMP assays.	Yes	Yes	Air-Dryable	Yes	No	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Air-Dryable DNA LAMP	DNA	LAMP	MDX119	Order a Sample	Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots	Glycerol-free mix for isothermal applications such as LAMP, containing Bst DNA polymerase, reaction buffer, dNTPs and air-dryable excipients, ideal for developing ambient temperature, LAMP assays.	Yes	Yes	Air-Dryable	Yes	No	4x	Yes	View Details		Certifate of Analysis	User Guide	Product Handling Guide
Air-Dryable Direct RNA/DNA qPCR Blood, 4x	Blood, Plasma, Serum, RNA	RT-qPCR	MDX121	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.	Yes	Yes	Air-Dryable	Yes	No	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready Direct DNA qPCR Blood, 4x	Blood, Plasma, Serum, DNA	qPCR	MDX122	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 20% whole blood, 20% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready Direct RNA/DNA qPCR Blood, 4x	Blood, Plasma, Serum, RNA	RT-qPCR	MDX123	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready Direct DNA LAMP Blood, 4x	Blood, Plasma, Serum, DNA	LAMP	MDX124	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 20% whole blood, 20% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.	Yes	Yes	Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
55C MMLV-RT	RNA	RT-qPCR, RT-LAMP	MDX117	Order a Sample	Available in 50 µL (10,000 Units), 200 µL (40,000 Units) or 1 mL (200,000 Units) aliquot	Highly robust, thermostable reverse transcriptase, ideal for cDNA synthesis from RNA with a high secondary structure such as viral RNA genomes.	Yes	Yes	Requires excipients	Yes	Yes	200 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Lyo-Ready 1-Step RT-qPCR Virus Mix	RNA	RT-qPCR	MDX062	Order a Sample	Available in 10 mL (1,000 Rxns) or 100 mL (10,000 Rxns) aliquots	Taq polymerase, RNase inhibitor, reaction buffer, dNTP, MgCl2 and lyo-excipients and separate reverse transcriptase, optimized for amplification of RNA with high secondary structure.	Yes	Yes	Lyo-Ready	Yes	Yes	2x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready qPCR Buffer w/o Excipients, 4x	DNA	qPCR	MDX061	Order a Sample	Available in 2.5 mL (500 Rxns) or 100 mL (20,000 Rxns) aliquots	Glycerol-free qPCR reaction buffer that is designed to allow formulation optimization to freeze-drying methods by excipients selection.	Yes	Yes	Air-Dryable,Lyo-Ready	Yes	Yes	4x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready 1-Step RT-qPCR Buffer	RNA	RT-qPCR	MDX052	Order a Sample	Available in 10 mL (1,000 Rxns) or 100 mL (10,000 Rxns) aliquots	Glycerol-free RT-qPCR reaction buffer containing lyo-excipients, designed to deliver highly reproducible, accurate RT-qPCR assay results under fast thermal cycling conditions.	Yes	Yes	Lyo-Ready	Yes	Yes	2x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Compatible MMLV-RT	RNA	RT-qPCR	MDX042	Order a Sample	Available in 8 µL (1,000 Rxns) or 80 µL (10,000 Rxns) aliquots	Glycerol-free high concentration reverse transcriptase and dilution buffer. Can be used to create 1-step RT-qPCR mixes for subsequent lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	> 165 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready 1-Step RT-qPCR Mix	RNA	RT-qPCR	MDX024	Order a Sample	Available in 10 mL (1,000 Rxns) or 100 mL (10,000 Rxns) aliquots	Glycerol-free Taq polymerase, reaction buffer, dNTP, MgCl2, and lyo-excipients, and separate thermostable reverse transcriptase, ideal for developing room temperature, lyophilized, multiplex qPCR assays.	Yes	Yes	Lyo-Ready	Yes	Yes	2x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
dATP, 100mM (Lithium)	DNA	qPCR	MDX046	Order a Sample	Available in 50 mL aliquots	Ultra-pure dATP (2-Deoxyadenosine-5-Triphosphate) lithium salt solution, enzymatically synthesized from premium quality raw materials.	Yes	Yes	Air-Dryable,Lyo-Ready	Yes	Yes	100 mM	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Lyo-Ready qPCR Mix 2.6x	DNA	qPCR	MDX023	Order a Sample	Available in 8 mL (500 Rxns) or 100 mL (12,500 Rxns) aliquots	Glycerol-free Taq polymerase, reaction buffer, dNTP, MgCl2, and lyo-excipients, ideal for developing room temperature, lyophilized, multiplex qPCR assays.	Yes	Yes	Lyo-Ready	Yes	Yes	2.6x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready qPCR Buffer, 2.5x	DNA	qPCR	MDX022	Order a Sample	Available in 8 mL (500 Rxns) or 100 mL (12,500 Rxns) aliquots	Glycerol-free qPCR reaction buffer containing lyo-excipients, designed to deliver highly reproducible, accurate qPCR assay results under fast thermal cycling conditions.	Yes	Yes	Lyo-Ready	Yes	Yes	2.5x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready qPCR Mix	DNA	qPCR	MDX021	Order a Sample	Available in 5 mL (500 Rxns) or 100 mL (10,000 Rxns) aliquots	Glycerol-free Taq polymerase, reaction buffer, dNTP, MgCl2, and lyo-excipients, ideal for developing room temperature, lyophilized, multiplex qPCR assays.	Yes	Yes	Lyo-Ready	Yes	Yes	2x	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
High Conc. Glycerol-Free Bst	DNA	LAMP	MDX018	Order a Sample	Available in 80 µL or 800 µL aliquots	High concentration glycerol-free DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification).	Yes	Yes	Requires excipients	Yes	Yes	100 units/mL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Aptamer Taq HS (Glycerol-Free)	DNA	PCR	MDX015	Order a Sample	Available in 20 ?L (1,000 Units) or 1 mL (50,000 Units) aliquots	Glycerol-free, high concentration hot-start Taq DNA polymerase, suitable for difficult, multiplex molecular tests that require fast amplification.	Yes	Yes	Requires excipients	Yes	Yes	50 U/mL	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Glycerol-Free Taq HS, 50 U/µL	DNA	PCR	MDX011	Order a Sample	Available in 0.02 mL (1,000 Units) or 0.5 mL (25,000 Units) aliquots	Lyophilization-compatible high-performance Taq DNA polymerase, separate Taq antibody and enzyme dilution buffer, suitable for difficult, multiplex tests that require fast amplification, whilst maintaining high specificity and sensitivity.	Yes	Yes	Requires excipients	Yes	Yes	50 U/mL	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Lyo-Ready Direct RNA NASBA Blood	Blood, Plasma, Serum, RNA	NASBA	MDX220	Order a Sample	Available in 400 mL (50 Rxns) or 2 mL (250 Rxns) aliquots	Single pot, one-step Nucleic Acid Sequence-Based Amplification (NASBA) for reliable isothermal amplification of RNA transcripts from crude or purified blood samples, ideal for POC testing	Yes	Yes	Lyo-Ready	Yes	Yes	2.5x	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free DNA Helicase (HC)	DNA	LAMP, RPA, RCA, SDA	MDX241	Order a Sample	Available in 20 ?L (1 µg) or 200 ?L (10 µg) aliquots	Glycerol-free, high concentration enzyme used to unwind double-stranded DNA with the help of an accessory protein, allowing you to deliver reliable results with the added capability for lyophilization. MutL	Yes	Yes	Requires excipients	Yes	Yes	50 µg/mL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Lyo-Ready Direct RNA NASBA Urine	Urine, RNA	NASBA	MDX223	Order a Sample	Available in 400 mL (50 Rxns) or 2 mL (250 Rxns) aliquots	Single pot, one-step Nucleic Acid Sequence-Based Amplification (NASBA) for reliable isothermal amplification of RNA transcripts from crude or purified urine samples, ideal for POC testing	Yes	Yes	Lyo-Ready	Yes	Yes	2.5x	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Lyo-Ready Direct RNA NASBA Stool	Stool, RNA	NASBA	MDX222	Order a Sample	Available in 400 mL (50 Rxns) or 2 mL (250 Rxns) aliquots	Single pot, one-step Nucleic Acid Sequence-Based Amplification (NASBA) for reliable isothermal amplification of RNA transcripts from crude or purified stool samples, ideal for POC testing	Yes	Yes	Lyo-Ready	Yes	Yes	2.5x	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Lyo-Ready Direct RNA NASBA Saliva	Saliva, Sputum, RNA	NASBA	MDX221	Order a Sample	Available in 400 mL (50 Rxns) or 2 mL (250 Rxns) aliquots	Single pot, one-step Nucleic Acid Sequence-Based Amplification (NASBA) for reliable isothermal amplification of RNA transcripts from crude or purified saliva samples, ideal for POC testing	Yes	Yes	Lyo-Ready	Yes	Yes	2.5x	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide


Title
Specimen Type
Application
Catalog
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Wet(Liquid)
Air-Dryable
Glycerol-Free
Lyo-Ready
Concentration
High Concentration
Description






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Superior Reproducibility, Sensitivity and Specificity

An ideal lyophilization formulation, for example, should stabilize an enzyme in a freeze-dried format and allow very fast rehydration and reactivation of the enzyme preparations without impacting its performance post rehydration. An ideal formulation should stabilize an enzyme in a freeze-dried or air-dried format and allow fast rehydration and reactivation of the enzyme preparations without impacting its performance post rehydration.

Meridian offers general and sample specific master mixes and glycerol-free enzymes and buffers for the preparation of both lyophilization and air-drying of your assays.

Advanced Enzyme Solutions for Molecular Biology Applications

How to Air-Dry an Assay

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Meridian offers diagnostic manufacturers the complete solution with over 3,000 antigens and antibodies covering more than 500 diseases and an extensive range of specialty qPCR/RT-qPCR master mixes and molecular enzymes all manufactured under ISO13485 regulations.

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Master Mixes for Molecular Ambient-Temperature Stable Assays

Master Mixes for Molecular Ambient-Temperature Stable Assays

Air-Dryable Mixes

Air-Dryable Mixes

FAQs: Important Principles for Air-Drying Molecular Assays

Can I use the Lyo-Ready mix for air-drying?

No, air-drying requires specific stabilizers, excipients, and preservatives which are different from those required for lyophilization.

Is it possible to adjust the oven temperature and do high temperatures damage the polymerase or reverse-transcriptase?

Yes, we recommend optimizing the oven temperature and drying time for each new assay formulation (mix including primers and probes), vessel used and lot size. High drying temperatures may affect the integrity of the mix and its components, including enzymes.

How do you know once drying is complete?

Ideally, 70% moisture should be removed from Air-Dryable™ 1-step RT-qPCR Mix (MDX095) and 95% from the Air-Dryable™ Mix (MDX082). However, the optimal moisture loss requires optimization for each assay. Retaining too much moisture may impact the shelf-life of the assay and over-drying may make the mixture difficult to rehydrate and cause a loss of performance. Moisture loss can be calculated by comparing the weight of the initial wet mix in the vessel to the dry mix (refer to the basic workflow for further information). Please note that the air-dried material must be packaged immediately after the drying cycle.

Is it easy to rehydrate an air-dried mix? Do you need to vortex?

The air-dried mix rehydrates in seconds after the addition of the sample. We do recommend, if possible, to gently shake/mix the vessels in order to resuspend the reaction mix before running the reaction, or alternatively mix the solution when adding the patient sample.

Why do I sometimes see a lower Ct value when I compare the performance of the re-hydrated dry mix against the wet mix?

If a mix becomes over-dried, the integrity of the enzymes will be compromised. Conceptually, the ideal “dryness” of a mix will be the highest percentage of moisture loss achieved without losing assay performance. If the Ct value of your dry mix is lower to the comparable wet mix, then the mix has been over-dried and further optimization is required.

Do different probes affect the performance of the assay after the mix is air-dried?

We did not observe any effect of the fluorophore type (Cy5, FAM, JOE or ROX) on the performance of the mix after air-drying.

Will the air circulation from the fan in the oven cause contamination?

We did not observe any contamination during air-drying however we do recommend good laboratory cleaning practices to minimize possible environmental contamination. Examples include surface cleaning or allocating separate locations for the oven drying, qPCR reaction setup, and analysis. In addition, we recommend maintaining the oven following the cleaning instructions provided by the manufacturer.

Could the fan air bring traces of primers/probes from one tube during the drying to another tube?

We did not observe any cross-contamination from fan-forced oven drying. Given the mix resides at the bottom of the vessel, it is very unlikely that cross-contamination can occur between the wells. In addition, in accordance with good manufacturing practices, we would discourage oven drying two different types of assay in the same drying cycle.

Do I have to use 8-well PCR tube strips?

Other vessels can be used such as plates, vials or various microchips, but the drying conditions for each vessel/assay would need to be optimized. Once the ideal conditions and parameters are established, they would need to be verified during validation and scale up.

Do you have a list of ovens that you recommend?

We recommend using a precision drying oven (temperature uniformity of +/- 1.5°C) with convection, fan-forced or vacuum capabilities. In our testing, we used a Memmert UF260Plus oven with fan and air flap settings at 100% and 20% respectively. Please note that different ovens will have different specifications and adjustable parameters that will need to be optimized based your assay, reaction volume, vessel type, batch size, relative humidity, and altitude.

Can I simply put the tubes back in the oven for longer incubation if the moisture loss is not enough, or add water if it is too much?

If the first attempt is not successful and does not provide the correct moisture loss value, we do not recommend putting the tubes back in the oven for additional drying or adding water. We recommend re-starting the experiment in order to control all of the variables so that they are reproducible. In addition, opening the door during the drying process is also not recommended unless it is done in a controllable manner and will be part of the process moving forward.

Would fluctuations in ambient humidity and temperature where the drying oven is located affect the drying protocol, such that the moisture lost is outside the 70% range?

Unlike the effects with lyophilization, there is no impact of the environment to the moisture loss with air-drying.

Can I test using different vessel types and/or different reaction volumes per well in the same drying cycle?

We recommend that different drying cycles are performed to test the effect of altering any assay variable such as vessel type, reaction volume, primer/probes, and lot size. This will ensure that the conditions and outcomes are reproducible.

Does the balance need to be next to the oven for quick weighing?

No, the balance does not have to be next to the oven, just close the vessel once it is out of the oven and go to the balance (even if in a different room) for measurement.

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Lyo-Ready & Air-Dryable Master Mixes & Reagents

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